Home Brew Forums > Home Brewing Beer > General Techniques > Guide to Making a Frozen Yeast Bank
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Old 07-27-2011, 11:58 AM   #521
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Are there any larger autoclavable vials than in the op? I'd rather not have to do two starters to get a frozen vial ready to pitch.

Or would a well stirred starter on a stirplate with one of these vials allow for good propagation?
You would need a pretty huge vial of frozen culture to get by with just a single step. Based on some vaguely scientific experiments with a hemocytometer, I think just two steps is even pushing it.


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Old 07-27-2011, 06:33 PM   #522
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I was thinking it'd be possible to freeze a good amount in a vial like White Labs sells theirs in (a good 30ml? whatever the amount is they sell). However, these aren't autoclavable.


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Old 07-27-2011, 07:06 PM   #523
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However, these aren't autoclavable.
Are you sure about that?
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Old 07-27-2011, 07:15 PM   #524
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Are you sure about that?
No, but they're made of plastic, as they're just 2L pre forms. So I would assume that they aren't.
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Old 07-27-2011, 07:18 PM   #525
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No, but they're made of plastic, as they're just 2L pre forms. So I would assume that they aren't.
How does White Labs sterilize them?

I read several years ago (I think on another forum) that they could be autoclaved.
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Old 07-27-2011, 09:51 PM   #526
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According to this thread: http://www.thebrewingnetwork.com/for...t=7769&start=8 you can't autoclave them. I have no idea how I'd sterilize them, which is why I ask if there's bigger vials that are autoclavable. Granted a starsan soak could be effective, but I don't want to risk losing a batch of beer.
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Old 07-28-2011, 01:46 PM   #527
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This post was also in that thread:

"by oneal66 Wed Feb 06, 2008 5:01 pm

In the interest of science I went ahead and used our autoclave here in the lab to check. White labs vials are autoclavable. I did a cycle @ 121C for 20 min, and the tube and cap both came out fine. However, the tube did come out discolored. I'm not sure if this would cause a problem with yeast health or not. The tube did not come out disfigured though.
oneal66"

White Labs may have their own (financial) reasons for saying that they're not autoclavable.
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Old 07-28-2011, 02:18 PM   #528
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Quote:
Originally Posted by bja View Post
This post was also in that thread:

"by oneal66 Wed Feb 06, 2008 5:01 pm

In the interest of science I went ahead and used our autoclave here in the lab to check. White labs vials are autoclavable. I did a cycle @ 121C for 20 min, and the tube and cap both came out fine. However, the tube did come out discolored. I'm not sure if this would cause a problem with yeast health or not. The tube did not come out disfigured though.
oneal66"

White Labs may have their own (financial) reasons for saying that they're not autoclavable.
No need to be cynical about Chris White's motives here, especially considering how incredibly positive a figure he has been in the HB community. I assure you, he is not worried about how your reusing his vials will affect his bottom line.

If a plastic vial changes color during autoclaving, it has undergone some kind of chemical or structural change. That means it is not autoclavable. Maybe it matters, maybe it doesn't (and maybe it won't matter until you've done it six or eight times), but for something to be properly called "autoclavable" it needs to remain stable through the process.
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Old 07-28-2011, 03:01 PM   #529
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They probably use UV light to sterilize the White Labs vials. Just a guess.
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Old 07-28-2011, 03:03 PM   #530
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Oh, and an obvious source of truly autoclavable larger volume storage: http://goo.gl/J8Ua5

Various sizes are available


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