Guide to Making a Frozen Yeast Bank - Page 52 - Home Brew Forums
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Old 05-14-2011, 01:57 PM   #511
MalFet
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Quote:
Originally Posted by orangehero
What do you recommend to transfer the yeast? I bough a 10 mL Mohr pipette, but the opening is too narrow to suck up yeast slurry. I'm thinking about getting a stainless turkey baster with a screw-on needle tip, but the bulb is probably not autoclavable.
Many people use medical syringes with good results. They're cheap and generally auto-clavable.


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Old 05-14-2011, 08:18 PM   #512
theredben
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All pipette tips are large enough to pick up slurry. Hop bits and assorted trub may clog the tips, but the yeast will get through just fine.



 
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Old 05-15-2011, 01:18 PM   #513
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Bought an auto-pipet with pyrex tubes. I think its the best way to go about it as far as extracting yeast/glyerine
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Old 05-15-2011, 01:30 PM   #514
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Quote:
Originally Posted by Thundercougarfalconbird
Bought an auto-pipet with pyrex tubes. I think its the best way to go about it as far as extracting yeast/glyerine
What model and from where if you don't mind?

 
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Old 05-16-2011, 12:57 AM   #515
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Auto from amazon - http://www.amazon.com/PIPETTE-PUMP-1...5507380&sr=8-7

Tubes from northern brewer- http://www.northernbrewer.com/defaul...ipet-10ml.html
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Old 05-16-2011, 01:03 AM   #516
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Thanks.

 
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Old 05-31-2011, 04:22 AM   #517
Cosper123
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Thank you very much for such a well thought out and detailed post.

 
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Old 07-26-2011, 08:13 PM   #518
Jublin
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Are there any larger autoclavable vials than in the op? I'd rather not have to do two starters to get a frozen vial ready to pitch.

Or would a well stirred starter on a stirplate with one of these vials allow for good propagation?

 
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Old 07-27-2011, 10:45 AM   #519
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What do you mean by two starters?
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Old 07-27-2011, 11:31 AM   #520
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Quote:
Originally Posted by bja
What do you mean by two starters?
Probably meant step up starters to increase cell count.

Nobody has patience or time these days?



 
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