Guide to Making a Frozen Yeast Bank - Page 5 - Home Brew Forums
Home Brew Forums > Home Brewing Beer > General Techniques > Guide to Making a Frozen Yeast Bank

Reply
 
Thread Tools
Old 10-15-2007, 02:57 AM   #41
Philip1993
Recipes 
 
Jul 2007
Posts: 1,636
Liked 9 Times on 9 Posts


Quote:
Originally Posted by jdoiv
Ok, so I went by the LHBS this morning and picked up what I hope is the right stuff. If was Glycerin USP Finishing Formula from JD Carlson. Is this the right stuff?

Yep. padding

 
Reply With Quote
Old 10-15-2007, 02:17 PM   #42
camiller
Recipes 
 
Apr 2007
Omaha, NE
Posts: 1,901
Liked 32 Times on 32 Posts


Quote:
Originally Posted by jdoiv
If was Glycerin USP Finishing Formula from JD Carlson. ...

I Think that is LD Carlson. Since they sell it as an additive for wines and liqueurs it certainly should be safe for consumption.

 
Reply With Quote
Old 10-15-2007, 10:08 PM   #43
PintOfBitter
Recipes 
 
Feb 2007
USA
Posts: 1,154
Liked 11 Times on 10 Posts


Wow, this sounds like something I'd like to try. I don't have any of the glassware needed, but I was looking around on that Cynmar company's site, and it looks like the whole "kit" could be pieced together pretty cheap. Maybe I'll go for it.

For how many generations is a given yeast supposed to be good for? I'm sure this process shouldn't be repeated forever, right? It seems like I remember the magic number of 4 generations from somewhere...
__________________
-PoB

Maybe you can use these Grain, Hops, Yeast Reference Charts

 
Reply With Quote
Old 10-16-2007, 01:13 AM   #44
left field brewer
Recipes 
 
Jul 2007
Lansing, MI
Posts: 85
Liked 1 Times on 1 Posts


Thanks for the info. I'm just starting to get into yeast banking. Is that a Corning PC-353? I got the same one at the university surplus store for $5. Its already paid for itself after making just a couple of starters, and the amount of yeast it produces per volume is pretty sweet. Also a little off topic but how hard was it to tap a valve into the top of the pressure cooker(pretty thick)? I'd hate to ruin the nice new $90 pressure cooker I'm looking at.

 
Reply With Quote
Old 10-16-2007, 01:16 AM   #45
Philip1993
Recipes 
 
Jul 2007
Posts: 1,636
Liked 9 Times on 9 Posts


Quote:
Originally Posted by PintOfBitter
Wow, this sounds like something I'd like to try. I don't have any of the glassware needed, but I was looking around on that Cynmar company's site, and it looks like the whole "kit" could be pieced together pretty cheap. Maybe I'll go for it.
Scientific glassware is nice, but you can get everything you need at the grocery, just remove the food and wash. Of all the pieces I have, the two I like best came filled with apple juice and Ragu...

mavtitle Likes This 
Reply With Quote
Old 10-17-2007, 03:10 AM   #46
FlyGuy
 
FlyGuy's Avatar
Recipes 
 
Jan 2007
Calgary, Alberta
Posts: 3,605
Liked 178 Times on 51 Posts


Quote:
Originally Posted by left field brewer
Thanks for the info. I'm just starting to get into yeast banking. Is that a Corning PC-353? I got the same one at the university surplus store for $5. Its already paid for itself after making just a couple of starters, and the amount of yeast it produces per volume is pretty sweet. Also a little off topic but how hard was it to tap a valve into the top of the pressure cooker(pretty thick)? I'd hate to ruin the nice new $90 pressure cooker I'm looking at.
Yep, I got mine surplus at my university, too! Works great still.

Regarding tapping the valve, it was dead easy. But I would recommend to anyone to be very cautious when you do it. If it isn't threaded properly, you are just asking for serious trouble. To anyone buying a new pressure cooker, I would HIGHLY recommend finding one that has a pressure relief valve that unscrews -- then you can add a tee fitting, put the pressure relief valve back on, and then add your own needle valve for the steam outlet. Brewman ! has pics of his system (see top of post for links).

 
Reply With Quote
Old 10-17-2007, 08:01 PM   #47
MadWeezel
Recipes 
 
Jun 2006
Clive, Ia
Posts: 112

One may also look at getting a separatory Funnel with stopcock, After making your starter and removing from the stirplate, transfer your slurry into the stopcock, seal let settle then sterilize the stopcock tip before pouring into the vials.


1000ml

250ml


 
Reply With Quote
Old 10-26-2007, 03:30 PM   #48
Andy77
Recipes 
 
Oct 2007
Posts: 17

Just FYI, you can get anything you need from a scientific supply house like Fisher Scientific or VWR, I plan on making my frozen stock soon, you can get sterile tubes, anything you need.

 
Reply With Quote
Old 10-26-2007, 03:36 PM   #49
mrkristofo
 
mrkristofo's Avatar
Recipes 
 
Sep 2007
Behind the zion curtain
Posts: 922
Liked 6 Times on 6 Posts


Quote:
Originally Posted by MadWeezel
One may also look at getting a separatory Funnel with stopcock, After making your starter and removing from the stirplate, transfer your slurry into the stopcock, seal let settle then sterilize the stopcock tip before pouring into the vials.


1000ml

250ml

I can't believe I didn't think of this. Well done. Really, good form.
__________________
Coming Soon:
Primary: Stone Vertical Epic 08.08.08
Secondary: Oaked Arrogant Bastard
Conditioning: Dead Guy Ale clone, Double-Dubbel
Drinking: Not for a while

 
Reply With Quote
Old 11-09-2007, 02:34 PM   #50
jds
 
jds's Avatar
Recipes 
 
Oct 2007
Littleton, CO
Posts: 1,913
Liked 23 Times on 18 Posts


Quick question / addition:

I've seen these baby soda bottles online for a fairly low price. They're preforms for 2 l soda bottles.



It's a bit hard to tell from the photos, but they look to be about the size of the White Labs pitchable liquid yeasts. I'm planning on saving and freezing some yeast from my next batch. Anyhow, I'm thinking these might be a good size for both storing/freezing yeast, and possibly building a mini-starter in as well.

Has anybody used these for yeast storage or starters?

 
Reply With Quote


Reply
Thread Tools


Similar Threads
Thread Thread Starter Forum Replies Last Post
Frozen yeast bank with yeast cakes? mrfocus Recipes/Ingredients 6 05-07-2015 05:18 AM
Frozen Yeast Bank vs. Yeast Slanting shortyjacobs Fermentation & Yeast 11 09-24-2010 07:13 PM
Frozen yeast bank not freezing T-Hops General Techniques 6 03-17-2010 03:20 PM
Yeast Starter from frozen yeast bank dfohio General Techniques 5 04-30-2009 07:32 PM
Frozen Yeast Bank- Strain Exchange? flyangler18 General Techniques 37 03-25-2009 01:17 PM


Forum Jump