Guide to Making a Frozen Yeast Bank - Page 2 - Home Brew Forums

Register Now!
Home Brew Forums > Home Brewing Beer > General Techniques > Guide to Making a Frozen Yeast Bank

Reply
 
Thread Tools
Old 08-09-2007, 11:48 AM   #11
thebikingengineer
Recipes 
 
Apr 2007
Redlands, California
Posts: 207
Liked 1 Times on 1 Posts


Any tips on sources for the vials? I'd like to start doing this, but I haven't been able to find these things except by the case.

 
Reply With Quote
Old 08-09-2007, 12:37 PM   #12
Chris_K
 
Chris_K's Avatar
Recipes 
 
Oct 2006
Chicago, IL
Posts: 97
Liked 2 Times on 2 Posts


Here's a dozen of the vials in 16ml for $6

Cynmar
__________________
Primary #1-Oatmeal Stout
Primary #2- Empty
Secondary #1- Empty
Kegged- Cream Ale
Someday/ Maybe/ Getting There- 'California Common', Impromptu IPA, Imperial Stout

 
Reply With Quote
Old 08-09-2007, 12:44 PM   #13
Bellybuster
HBT_SUPPORTER.png
 
Bellybuster's Avatar
Recipes 
 
Aug 2007
, Ontario, Canada
Posts: 905
Liked 161 Times on 126 Posts


I think this is really good that more HB'ers are doing this. I've been freezing yeast for a number of years now and prior to my recent move I had I think 7 different varieties in my "bank".
What I do is use the smack pack normally and freeze the bit that's left in the smack pack. I try to reuse the yeast cake whenever I can and if not I'll freeze 2 more vials from that. Each smack pack gives me 4 vials of frozen yeast and usually 3 batches on the yeast cake. That's 7 batches from the original $10 smack pack. Add in starter material and it works out to around $3 for each batch, a savings of around $7 every batch.
Once I had my yeast bank built up, I never again bought yeast
__________________
Beer me!!!!!!!
http://www.fawkesholebrewing.com

on Tap
FawkesHole "Guy"ness
FawkesHole I.R.A.
FawkesHole Powder Plot IPA

tharding Likes This 
Reply With Quote
Old 08-09-2007, 01:13 PM   #14
rdwj
 
rdwj's Avatar
Recipes 
 
Jun 2006
Plainfield, IL
Posts: 4,577
Liked 35 Times on 25 Posts


Quick question...

How often do you find yourself changing out the ice packs in your cooler? Would using a cooler small enough to fit in a freezer, and perhaps packing that with ice packs, stabalize the sample's temp enough to avoid the self-defrosting freezer issues?

..oh, and any idea how long a frozen sample will remain viable?

Thanks for the tutorial; it's very well done.
__________________
On Tap: Whatever I just brewed (got sick of updating it)


 
Reply With Quote
Old 08-09-2007, 01:17 PM   #15
FlyGuy
 
FlyGuy's Avatar
Recipes 
 
Jan 2007
Calgary, Alberta
Posts: 3,605
Liked 178 Times on 51 Posts


Quote:
Originally Posted by rdwj
Quick question...

How often do you find yourself changing out the ice packs in your cooler? Would using a cooler small enough to fit in a freezer, and perhaps packing that with ice packs, stabalize the sample's temp enough to avoid the self-defrosting freezer issues?

Thanks for the tutorial; it's very well done.
My pleasure on the write-up -- I have been promising it for a while now on a few previous threads where people have asked what I do.

Sorry, if it wasn't clear but the whole cooler goes into the freezer. The ice-packs are only there for temperature stabilization when the defrost cycle kicks in, as you suggest.

(I'll go back and edit that step just so it is really clear.)

 
Reply With Quote
Old 08-09-2007, 01:23 PM   #16
rdwj
 
rdwj's Avatar
Recipes 
 
Jun 2006
Plainfield, IL
Posts: 4,577
Liked 35 Times on 25 Posts


Quote:
Originally Posted by FlyGuy
My pleasure on the write-up -- I have been promising it for a while now on a few previous threads where people have asked what I do.

Sorry, if it wasn't clear but the whole cooler goes into the freezer. The ice-packs are only there for temperature stabilization when the defrost cycle kicks in, as you suggest.

(I'll go back and edit that step just so it is really clear.)

Ah, good! I'm only a few pieces of equipment away from doing exactly what you're showing here and I've been wanting to set up a steam injection system anyway. Now I have one more reason to get started.
__________________
On Tap: Whatever I just brewed (got sick of updating it)

 
Reply With Quote
Old 08-09-2007, 01:30 PM   #17
FlyGuy
 
FlyGuy's Avatar
Recipes 
 
Jan 2007
Calgary, Alberta
Posts: 3,605
Liked 178 Times on 51 Posts


Quote:
Originally Posted by thebikingengineer
Any tips on sources for the vials? I'd like to start doing this, but I haven't been able to find these things except by the case.
Some good deals can often be found on eBay. I almost bought some there. Actually, I got mine from my local university. You can phone their Chemistry Department and ask to speak to the person running their Chemistry Stores shop. Sometimes they will sell items to the public.

You shouldn't have to pay more than about 50 cents per vial. This may seem like a lot if you buy 30 or 40 of them, but if you get the glass autoclavable ones, they clean up easily and and you can sterilize them in the pressure cooker over and over. You can also find much cheaper vials out there, but they probably won't last as long.

 
Reply With Quote
Old 08-09-2007, 01:51 PM   #18
fifelee
 
fifelee's Avatar
Recipes 
 
Dec 2006
Vaughn, MT
Posts: 1,046
Liked 40 Times on 27 Posts


The best write up I have seen. Thanks. I have been apprehensive in the past because I couldn't work out some of the details, but I actually feel like I can do it now.

 
Reply With Quote
Old 08-09-2007, 01:53 PM   #19
98EXL
 
98EXL's Avatar
Recipes 
 
Mar 2007
Maryland
Posts: 3,138
Liked 14 Times on 14 Posts


very nice writeup! I've started more or less doing the same thing....just refrigerating for now...need some more stuff to freeze my babies
__________________
I'm back!!!

 
Reply With Quote
Old 08-09-2007, 04:22 PM   #20
Bellybuster
HBT_SUPPORTER.png
 
Bellybuster's Avatar
Recipes 
 
Aug 2007
, Ontario, Canada
Posts: 905
Liked 161 Times on 126 Posts


Not that Flyguys write up isn't good enough, it's excellent.
CD Pritchard put out another great article on freezing yeast. This is how I got started and the kit linked to on that site is a great cheap way to get started without layin out much cash.

http://home.chattanooga.net/~cdp/fre...#Sources-maybe
__________________
Beer me!!!!!!!
http://www.fawkesholebrewing.com

on Tap
FawkesHole "Guy"ness
FawkesHole I.R.A.
FawkesHole Powder Plot IPA

 
Reply With Quote


Reply
Thread Tools


Similar Threads
Thread Thread Starter Forum Replies Last Post
Frozen yeast bank with yeast cakes? mrfocus Recipes/Ingredients 6 05-07-2015 05:18 AM
Frozen Yeast Bank vs. Yeast Slanting shortyjacobs Fermentation & Yeast 11 09-24-2010 07:13 PM
Frozen yeast bank not freezing T-Hops General Techniques 6 03-17-2010 03:20 PM
Yeast Starter from frozen yeast bank dfohio General Techniques 5 04-30-2009 07:32 PM
Frozen Yeast Bank- Strain Exchange? flyangler18 General Techniques 37 03-25-2009 01:17 PM


Forum Jump