Originally Posted by BeeGee
Dilute mashes result in less substrate for beta-amylase to latch onto, as well as the enzymes being less heat-stable and hence subject to denaturation. Like I said, efficiency may suffer some.
Yes, efficiency may suffer that you are 100% correct.
Dilute mashes result in a lower substrate density but not less substrate in a closed system unless you spill more in a dilute system and we would have to look at what order enzyme kinetics we are talking about to determine if there would be statistical difference.
The amalyase enzyme is amazingly stable and a dilute mash will not hurt the tertiary/quantinary structure. The half-life of beta-amylase in 18.2 M-ohm water is 10 years at -20 d c, >72 hours at 37 d c and > 24 h at 75 deg C.* I could go on, but it can be boring! However, one cool study showed the stability of alpha and beta-amalyase being nearly 3 months at 70deg C on a solid phase system.
Efficiency will suffer but not due to enzyme stability or denaturation. Efficiency may suffer due to overall substrate concentration but minimally. We could always do a Eadie-Hofstee plot if you want to have fun (more accurate than Lineweaver Burke plots)
* following standard scientific equations of:
Half live= ln2/kd where kd is the deactivation constant