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Old 04-05-2012, 01:03 PM   #41
edecambra
 
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Banking yeast on agar slants, at least that's what I'm doing.

 
Old 04-05-2012, 01:08 PM   #42
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That is my plan too.
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Old 04-05-2012, 01:13 PM   #43
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Quote:
Originally Posted by crazyseany
what are theses being used for? freezing yeast?
That's my plan. I want to make a frozen yeast bank to save all of the strains I use

 
Old 04-05-2012, 02:16 PM   #44
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I havnt decided if I'm doing slants or frozen bank yet, more reading to do still
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Old 04-05-2012, 05:32 PM   #45
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I plan on doing a frozen bank once I get my tubes I will pick up some food grade glycerin and hopefully my centrifuge off ebay. Then spin out most of the water add some glycerin and slowly freeze it. I figure if I have 5 different types of yeast and 5 vials of each strain. I can brew what I want and not have to buy yeast for years. Use one vial 5 to 10 time each time washing the yeast cake. then move to the next vial of that yeast rinse and repeat. Once I use up that vial I will then have 2 vials of generation 2 then use up both those go to then next vial rinse and repeat. I know there is math there but I a to lazy to figure it at the moment

It is a lot of batches though.
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Old 04-05-2012, 05:44 PM   #46
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You can do the same thing buy flooding the slant with glycerin. Streak the slant like normal then let it grow for a few days until you get some nice big colonies. Flood it with glycerin. One thing is you need to freeze as fast as you can. slow freezing makes huge ice crystals that kill the yeast. When I buy a Tube or packet of yeast I use it to streak 10 to 20 slants then use the rest to make a starter. I figure I can get about 100 ten gallon batches out of one tube or pack. When I am ready to make a batch I take a slant out of the freezer and into the fridge once it is thawed I will dump most of the glycerin and use a bit of starter wort to rinse the yeast off the slant. That goes into a 250ml starter that is in a 500ml flask. After a day spent spinning I add 100ml more starter and let that go for 12 hours. At that point you can stop or most times I go into a 1000ml or 1500ml starter from there. After you do that once you can use the yeast cake washed or not for up to 10 generations with out re-culturing it.
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Old 04-05-2012, 05:55 PM   #47
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Signed up for 25

 
Old 04-05-2012, 06:13 PM   #48
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Oh forgot to let everyone know the sheet is working now just had to change a setting.
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Old 04-06-2012, 02:27 AM   #49
konrad
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Added 25 to the order.

What are you doing for petri dishes?

 
Old 04-06-2012, 02:35 AM   #50
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I could look in to petri dishes. Would anyone be interested in pre made and sterilized slants?
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