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Old 02-19-2012, 12:29 AM   #1
Feb 2011
Culpeper, Va
Posts: 411
Liked 21 Times on 18 Posts

Just noticed mr malty said to do a 2L starter with two packs..

I did a 2L with one pack..

Mr Malty says I'll need 422B cells.

This chart says a 2L starter on a stir plate will produce 400B


(it says to double these numbers for stir plates)

Why would I need to pitch two packs?
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Old 02-19-2012, 01:32 AM   #2
Jul 2010
Pflugerville, Texas
Posts: 347
Liked 13 Times on 13 Posts

If you look at the calculator on, you can see what volume of starter you need to get 422 billion from 1 vial (100 billion per vial) and stirplate. You'll find that you'll need close to 4 L to get the population growth to hit your target. Mrmalty tends to optimize for smaller starter volumes by bumping the initial size of yeast. That is, you can get to 422 with 2 vials with less starter volume.

To be fair, I haven't found any studies that have formulas for determining yeast growth with stir plates to see how those two calculators arrive at thier growth projections, but I have confirmed that they both agree with starter sizes if you match the number of vials in mrmalty into the initial pitch size at
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Old 02-19-2012, 02:57 AM   #3
Dec 2011
l.a., ca
Posts: 1,372
Liked 14 Times on 14 Posts

422 bill is a gang of cells I guess you will step it to get that much.

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