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Old 09-19-2011, 03:23 PM   #11
ReverseApacheMaster
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I have some yeast cultures frozen in my parents' chest freezer. I haven't pulled one to see if it is viable but I assume as long as they never thawed they should be fine. I'll have to pull a sample next time I visit and see how it works out.

 
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Old 09-19-2011, 03:54 PM   #12
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Quote:
Originally Posted by ReverseApacheMaster View Post
I have some yeast cultures frozen in my parents' chest freezer. I haven't pulled one to see if it is viable but I assume as long as they never thawed they should be fine. I'll have to pull a sample next time I visit and see how it works out.
I'm willing to bet that they are still alive.
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Never Ending Liquid Yeast - How to Farm Yeast and Freeze it.


 
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Old 10-01-2011, 06:06 PM   #13
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Just a small update. I just got done freezing my yeast for the year and decided to do a little qualitative testing while I was at it. I used three different strenght glycerine solutions this time. 35% 50% and 60%. My plan is to see if different levels of glycerine affect the lag time when making the first step of starter from frozen yeast. If more glycerine = shorter lag time then I will assume that more glycerine = more viable yeast surviving the freeze. If I observe no appreciable difference, then I will stay with my current assumption that the yeast are just sluggish after freezing and need more time to regain activity. If Brewitt does some viability assays for us and contributes that information, then we should have a pretty good idea of what to expect from freezing yeast at home.
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Old 10-01-2011, 06:52 PM   #14
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Thank you for the great write up! This is very tempting, hehehe....

 
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Old 10-01-2011, 07:03 PM   #15
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Got my pressure cooker this week with some glycerine.
Just finished testing it by canning 7 qts of water and pressure cooking it (I will now have sterile water on hand for a while). I also did a 50:50 glycerine/water in 2x 4oz jars (only filled halfway, so yeast can occupy the other half). Next weekend I will be bottling and I plan on freezing washed yeast.

Will 25% glycerin be enough to keep them viable?

 
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Old 10-01-2011, 07:08 PM   #16
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Try it and find out. I'm guessing it will since I've heard reports of people using 15%.
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Old 10-01-2011, 07:33 PM   #17
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Awesome writeup and I look forward to following this post.
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Old 10-03-2011, 05:28 AM   #18
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Quote:
Originally Posted by BBL_Brewer View Post
If Brewitt does some viability assays for us and contributes that information, then we should have a pretty good idea of what to expect from freezing yeast at home.
Sorry for the delay in doing some experimentation on this. Work has been quite busy and I have had no time for side projects. I will prep a starter this week and set up some frozen preparations at a few glycerin concentrations from 15 to 60% and at -30C and -80C. It will probably be a couple weeks before I know anything about viability since it requires cell counts and colony counts.

 
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Old 10-03-2011, 05:44 AM   #19
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That would be great!
I had good results with 30% glycerin solution, and I think that success with smaller concentration will depend on how well is glycerin mixed with slurry.. but experiment will give more relevant informations about that.

 
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Old 10-03-2011, 06:13 AM   #20
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i had good results with 30% glycerin solution
+1

 
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