Hydrogen peroxide for agar plates/slants? - Home Brew Forums
Register Now For Free!

Home Brew Forums > Home Brewing Beer > Fermentation & Yeast > Hydrogen peroxide for agar plates/slants?

Thread Tools
Old 02-15-2011, 03:44 PM   #1
Jul 2008
Auburn, New York
Posts: 85

I have seen small amounts of hydrogen peroxide mixed in with potato dextrose agar when working under less than ideal conditions for mycology use. The theory was that living organisms (the mycelium in this case) could process the peroxide in small concentrations, while spores would be destroyed, and in practice, this led to a tremendous decrease in contamination when working without a glove box or flowhood. Does anyone have experience trying this with malt agar in their plates or slants?

Reply With Quote
Old 02-15-2011, 08:06 PM   #2
dinertime's Avatar
May 2009
New York, NY
Posts: 76
Liked 1 Times on 1 Posts

I have never heard of that, though all of my lab work has been done in what I assume could be considered close to "ideal" conditions. Do you have any information on the concentration of H2O2? I think I would rather try to be extra careful and/or make replicates of my plates and slants than risk oxidative damage of the yeast.
Ferm 1: ESB
Ferm 2: Chocolate Wheat
Drinking: Blocktoberfest

Reply With Quote
Old 02-15-2011, 09:25 PM   #3
Dec 2010
Langley, BC
Posts: 934
Liked 24 Times on 23 Posts

Are you having problems with contamination? Sanitizing working surfaces, closing all windows and door, and using a heat source to create an updraft is usually sufficient for us.
I would think it would be difficult to accurately measure such a small amount of H2O2 given the small amount (200mL) of media I usually make.

Reply With Quote
Thread Tools

Similar Threads
Thread Thread Starter Forum Replies Last Post
Universal Beer Agar? FrostyBeverage Fermentation & Yeast 8 09-24-2014 02:03 PM
Condensation problems on slants and plates Budzu Fermentation & Yeast 5 09-24-2010 12:26 PM
Malt extract agar powder? ekjohns Fermentation & Yeast 2 08-20-2010 08:11 PM
Proper agar recipe Budzu Fermentation & Yeast 3 03-03-2010 05:59 PM
Innoculated Refirgerated Agar Slant shelf life Sawdustguy Fermentation & Yeast 6 09-03-2009 02:24 PM

Forum Jump