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Old 09-09-2010, 02:26 PM   #1
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Jun 2010
Posts: 83

I recently happened upon a vine of hops near my home. They look big plump and healthy and have a wonderful fresh citrusy aroma (akin to cascades), and I would like to use them for an all-locally grown IPA. I will definitely use large quantities of them near the end of the boil, but is there any way to estimate their bittering potential? Maybe a small test batch on the stove?

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Old 09-09-2010, 03:11 PM   #2
david_42's Avatar
Oct 2005
Oak Grove, Oregon, USA
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Make a liter of hop tea using 30 g of the wild hops (wet, 60 minutes) and another liter from a known high AA hop (use 5 g). Compare the bitterness, dilute the known hop tea until it compares. 10 IBU is about the threshold for most people to make accurate comparisons.

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Old 09-09-2010, 03:15 PM   #3
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Jun 2010
Carnegie, PA
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This is a question that has vexed me every time I've considered growing my own...I'd be afraid to use them for anything other than aroma or dry hops. I've supposed you could just use the "average" alpha rating for a given variety, but that leaves a wide margin of error when you're talking bittering.

It'd be cool if someone sold an affordable home test kit for alpha acids...

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Old 09-09-2010, 06:21 PM   #4
Oct 2007
Lafayette, IN
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From BYO an article on how to measure IBUs of beer. All you need is a UV/VIS spectrophotometer. Most high school and all college chem and bio lab will have several that they might let you use in exchange for a six pack.

The formula is IBUs = 50 * (the absorbence at 275nm)
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Old 09-09-2010, 06:29 PM   #5
Jan 2008
Drain, OR
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Shoot. They run about 4k new. Someone could buy one and then charge home hop growers 10$ a pop to check their AA's for them. You might make your money back after a few years! :P
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Old 09-09-2010, 06:39 PM   #6
Aug 2009
Twin Cities, MN
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Originally Posted by effigyoffaith View Post
From BYO an article on how to measure IBUs of beer. All you need is a UV/VIS spectrophotometer. Most high school and all college chem and bio lab will have several that they might let you use in exchange for a six pack.

The formula is IBUs = 50 * (the absorbence at 275nm)
The article isn't clear on the procedure says a "simple solvent extraction is used", and since it's talking IBUs I'm guessing it's a liquid-liquid extraction. The details aren't specified however. I think it is more complicated then just a few drops of beer in your UV/VIS spectrometer though.

Hmm, some Googling turned this procedure up from some chem lab:

Student sheet: Bitterness of beer

The determination of the bitter substances in beer, which are mainly iso-R-acids (with a five member ring) originating from R-acids present in hops.

Bitterness is an essential quality parameter in modern breweries, and analysis of bitterness in beer and wort is conducted as a routine throughout the brewing industry. The traditional and international recommended analysis of bitterness in beer, in international bitter units (IBU), is carried out by a spectrophotometric measurement at 275 nm of an acidic solvent extract of beer. Acidified beer is
extracted with 2,2,4-trimethyl pentane. The bitter substances dissolve in the organic phase. After centrifugation, the absorbance of the 2,2,4-trimethyl pentane phase is measured at 275 nm against pure 2,2,4-trimethyl pentane.

Reagent solutions
hydrochloric acid, 6 mol/L


Degas the beer samples by gently stirring with a magnetic stirrer on low speed. Degassing agents and filtration may decrease the bitterness and is therefore not recommended. Plastic containers must not be used.

Iso-R-acids adsorb onto glass. Rinse thoroughly cleaned and/or new glassware first with an 2,2,4-trimethyl pentane beer extract before use.

Pipette 10.0 mL degassed beer into a 35 mL centrifuge tube or a 50 mL conical flask. Add 0.5 mL HCl (6 mol/L) and 20 mL 2,2,4-trimethyl pentane. Place 2 to 3 glass balls in the tube. Screw a cap on the centrifuge tube or stopper the flask, to get a solvent tight seal. Shake the centrifuge tubes for 15 minutes at 20 1 C in a rotary shaker set at 130 5 rpm or use a platform shaker and shake 20 1 C until a maximum extraction has been achieved, or shake by hand. Measure the absorbance of the 2,2,4-trimethyl pentane phase at 275 nm at intervals during shaking until no further increase in absorbance is observed, to establish the time of shaking.

Allow the emulsion to settle, centrifuge the tubes for 3 minutes at 3000 rpm (or use a separation funnel).
Measure the absorbance of the 2,2,4-trimethyl pentane phase in a 10 mm cuvette at 275 nm, using pure 2,2,4-trimethyl pentane as a reference. Before measuring, the cuvette must be washed with a part of the 2,2,4-trimethyl pentane phase. Record the sample absorbance in a few seconds. Absorbance measurement should be made within 20 minutes after centrifugation (or separation in the funnel) because further delay will affect the results.

Bitterness (IBU) = 50 A275

where A275 is the absorbance at 275 nm measured against 2,2,4-trimethyl pentane as a reference.

Source (Warning: PDF)
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Old 09-09-2010, 06:50 PM   #7
Aug 2010
Posts: 33

if you are using them toward the end of your boil you won't get much bitterness anyway. I would probably use a little dme and calculate down to a super small batch and boil for 60 minutes and taste after it cools. Just remember that around 5 ounces of wet hops is equal to around 1 ounce of dried hops.

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