Home Brew Forums > Home Brewing Beer > Fermentation & Yeast > throughts on starter step ups from frozen yeast
Thread Tools
Old 08-20-2010, 03:40 PM   #1
Feedback Score: 3 reviews
Join Date: May 2009
Location: IL
Posts: 1,400
Liked 22 Times on 20 Posts
Likes Given: 1

Default throughts on starter step ups from frozen yeast

I have access to a -80C fridge and was planning on starting a yeast bank. What I would do is upon opening a white labs tube or wyeast smack pack freeze down 20 vials of 1ml of yeast with an additional 300 ul glycerine. I would like to use the least amount of step ups to get to a final of 2L along with the least amount of use on a borrowed pressure cooker. What would be the easiest way to do this?

ekjohns is online now
Reply With Quote
Old 08-21-2010, 04:42 AM   #2
Feedback Score: 1 reviews
Join Date: Sep 2006
Location: Sacramento, CA
Posts: 360
Liked 7 Times on 5 Posts
Likes Given: 4


It's a long article, but I'd suggest you read this:


The article speaks about building up pitches from very small colonies of yeast. Hopefully it helps.
cdburg is offline
Reply With Quote
Old 08-21-2010, 05:51 AM   #3
Feedback Score: 0 reviews
Join Date: Jun 2010
Location: Melbourne, Australia
Posts: 95

My procedure is something like this, not the 'least amount of steps' but rather a slow, sure and safe way:
Frozen yeast, slant, 5ml, 20ml, 100ml, 500ml, 2L

The slant allows me to visually check the yeast viability after coming out of the freezer, then the small steps help 'proof' the yeast, keep any infection sources under control and hopefully get maximum growth and health from the yeast at each step.

In theory with sterile conditions/media you could go directly from the freezer to the 2L starter, or any number of intermediate steps, if the only criteria was 'least number of steps'. However, depending on what/where you read, steps of about 6-10x are usually recommended.
Wolfy is offline
Reply With Quote

Thread Tools

Similar Threads
Thread Thread Starter Forum Replies Last Post
Step up starter? jldc Fermentation & Yeast 1 07-20-2010 06:44 PM
Starter step up ratios??? artguy Fermentation & Yeast 13 06-16-2010 01:50 AM
Making a yeast starter - Step by Step Video johnnyc Fermentation & Yeast 2 02-05-2010 08:52 PM
2 step starter? mthhurley Fermentation & Yeast 1 01-29-2010 06:46 PM
Yeast starter wort frozen pjk49202 Fermentation & Yeast 5 12-10-2009 10:35 PM

Forum Jump