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Old 07-22-2010, 03:12 PM   #1
Islandboy85
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Default Long term yeast storage: agar vs. water

I'm trying to decide on the method for storage for my yeast library. I'm thinking about going with water instead of agar slants. From what I understand, water let's you store your cultures longer. Another benefit is that you do not have to refrigerate the culture as with an agar slant. The water slants can also be stored in smaller vials from what I read off one link off of Google. l Am I understanding this correctly?


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Old 07-22-2010, 03:21 PM   #2
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I have been researching this and have switched to using only distilled boiled water. According to the research of Dr. Emil Christian Hansen, he was able to store yeast in distilled de-oxygenized water for 14 years and have it still be viable.

Viability will certainly be an issue that you will have to learn how to deal with, however, ample time and a stir plate should allow you to culture up sufficient yeast to brew with. As in any long term yeast storage you can either use the craps shoot method and just culture up a starter and pray for the best or you can make a mini batch with the yeast.

I use yeast nutrients and a stir plate to get my yeast up and running.

m.


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Old 07-22-2010, 03:56 PM   #3
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My plan is to streak a plate, make a 10ml starter from spores off the plate. Then step it up to 50, 250, 500, 1000ml etc as needd for my cell count. I figure the plate will make it easy to get the best chance of a good. 10ml starter from the get go.
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Old 07-22-2010, 04:14 PM   #4
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Sounds like you have a good plan. Flame everything that could contaminate it.

I starsan spray everything, then flame the loop and the bottle/flask opening. Cover everything with aluminum foil that has been in starsan.

Seems like a lot of work to some people, but to save 8 dollars every brew is a great. I calculate it out to 15 cents a beer. I should have my yeast culturing equipment paid off in about 10 brews. After that is money in the bank!

But really its the fun of culturing the yeast.

m.
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Old 07-22-2010, 04:22 PM   #5
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I just bought a hemacytometer for $15 new on ebay. I'm getting a cheap Mead 900x microscope for $7. A local guy has shipping container full of lab glass from a shutdown refinery that I'm going to check out. He said he'll sell for 30% of new cost, so I'll get a lot of my beakers and erlenmeyer flasks off him. I still need to find a cheap place to get the culture tubes and a few glass petrie dishes.I'll need to buy an inoculation loop, and I need the pressure cooker too. I'll check craigslist for that. Not bad for a start. Pretty cheap really for the cost of a smack-pack or tube of yeast. Besides that, it's fun to be geeky and watch our cultures make bubbles.
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Old 07-23-2010, 02:39 AM   #6
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Do you think a microscope with 43x is enough to count my yeast?
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Old 07-23-2010, 02:44 AM   #7
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This is the type of scope I use. I only look at colonies on plates. Mine does 20x. So 43x should show a lot.

http://www.amazon.com/C-Scientific-D.../dp/B000Z4NYX6

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Old 07-23-2010, 06:11 AM   #8
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Quote:
Originally Posted by Mateo View Post
Sounds like you have a good plan. Flame everything that could contaminate it.

I starsan spray everything, then flame the loop and the bottle/flask opening. Cover everything with aluminum foil that has been in starsan.

Seems like a lot of work to some people, but to save 8 dollars every brew is a great. I calculate it out to 15 cents a beer. I should have my yeast culturing equipment paid off in about 10 brews. After that is money in the bank!

But really its the fun of culturing the yeast.

m.

In addition I think that beer from re-pitched yeast is actually better....
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Old 07-23-2010, 09:13 AM   #9
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Well, washing and storing that much yeast would take up too much space for my purpose. Plus I think after a few batches (from what I've read) your yeast mutates anyway. If I culture new yeast each time I'll avoid those problems. Plus, if I also understand yeast washing and storage, it's only viable for short term storage if you don't repitch it right off the bat. I hope I got all of this right.
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Old 07-23-2010, 03:25 PM   #10
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Mateo, what size vials do you use? And how many culture do you keep of a same yeast?


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