Home Brew Forums > Home Brewing Beer > General Techniques > Problems Storing Yeast
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Old 04-16-2010, 11:59 AM   #11
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Sweet, thanks for the info! The first few washings I did, that lasted for months, we made with boiled tap water.....Guess I need to go back to that!


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Old 04-19-2010, 04:23 PM   #12
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Originally Posted by Gremlyn1 View Post
Putting a cell into distilled water is putting it in a 'hyoptonic' environment. Basically, that means the distilled water has less solubilised molecules in it than the water contained inside the yeast cell. This creates a concentration gradient, and the distilled water will flow into the cell to try to balance the concentration gradient through osmosis.
Really? Then why doesn't the same thing happen when you use tap water? You are moving the yeast from the wort (1.010 gravity?) into a water solution (1.000 gravity). It seems to me that this situation would be just as bad as using distilled.

I've heard this same explanation used to describe what happens when marine fish are removed from a salt water environment (1.023 gravity) into a fresh water environment (1.000 gravity. But this is a drastic change.
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Old 04-19-2010, 08:18 PM   #13
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Tap water doesn't have a SG of 1.000, only pure water does. There are enough ions in tap water to keep osmosic activity to a minimum. The more pure the water, the more chance of engorging your yeast cells with H2O you have. Grab yourself a microscope and some DI water and see for yourself.

Here is a video of hemoglobin in distilled water:
(I know they aren't structurally the same, but the principle applies)
Originally Posted by Yooper View Post
I'm a fan of "getting it in the can"!
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