Originally Posted by timOD
I'm planning on harvesting some Bells yeast for a Two-Hearted clone and I'm wonder when you harvest, do you need to create new wort every time you step up? Or can I create all the wort I need in one flask on day one and just add it to gradually?
Easiest way to avoid the problem you allude to above is to make a batch of 1040ish wort and can it. I will typically do this about once a year and have five gallons of sterilized pint-sized starter wort to use throughout the year. Of course if I run out, I just plan a high gravity beer and after lautering batch it to sparge another lower gravity wort and top up with DME to about 1040, if necessary.
But if you do it the gradual-from-the-same-wort-way you mention I would be sure to boil and cool it again, but I am a little OCD sometimes about my wort. If you keep it sterile the entire time it is not fermenting you may be okay.