Yeast washing and pitching on cakes aside, does anyone grow one package of yeast enough for multiple brews?
I'm thinking of something like this:
Take a package of my favorite yeast and make a 1000ml starter in my 1000ml Erlenmeyer flask (EF), let grow. Transfer that to a growler and add another 800ml of wort and let that grow. Divide that 1800ml equally into 4 1000ml E-flasks (450ml each) and top them off with 550ml of wort and grow then refrigerate (or maybe wash and store in sterilized canning jars like the "yeast washing" thread).
Would this be enough yeast for pitching?
Could I use one of those 4 and do the same process over again and turn that 1000ml into 4 more starters? Could I take one of those 4 and do the same yet again?
How far can yeast be propagated before you run into issues with mutations, too many dead cells, off flavors, etc...????