I brewed a Dead Guy Ale clone about a week and a half ago, and used Rogue's Pacman yeast strain. Figuring it was on a limited release, and my love of Rogue's brews, I decided this would be a very good time to give yeast washing a try. I think it went pretty successful. I filled four one quart mason jars full, although the last one has a lot more trub in it than the other three, so I think I may dump that one.
Anyway, onto my question. What is the proper way to make a starter from washed yeast? I have a 1L erlenmyer flask that I do starters in, so pouring a whole quart in there isn't an option. Also, I assume I want to avoid putting as much trub in the starter as possible. So, what I'm wondering is, what is the most effective way to get the most yeast possible into the flask with the starter wort, while leaving as much of the other stuff behind?
Here's a picture of the washed yeast I plan on using:
I assume the white layer above the trub is what I want to get into the flask. How to get that while leaving the trub behind is where I'm stuck. I'm sort of thinking my only option is to get everything in the mason jar into suspension, and then pour some of the muddy liquid into the flask, and throw the remainder out. Or, is there some surefire method that I'm overlooking?