Originally Posted by Atticmonkey
I read in a few places about when it comes time to make a starter from a yeast slant that you can put sterile wort into your slant tube, swirl it around and pour it into your starter. Does anybody here do that? If so will that slant regrow itself or is it spent after that? Thanks for the advice.
That's the way I do it. I pour about 5ml of wort ontop of the slant and gently scrape off the yeast with an inoculation loop, if I do it well there is no yeast left on the slant so it's discarded.
However, some people use an inoculation loop to select only a small amount of yeast from the slant, and add that to a very small amount of wort. That way they don't 'use up' the slant and can continue to reuse the same slant for a long period of time (up to 4 years in some cases), however it does take an extra step when making the starter.