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Old 10-27-2009, 11:00 PM   #1
krops13
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I know there was a post recently asking for pictures of starters. But that's not what im looking for. Does anyone have pictures of starters built up from a culture?

 
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Old 10-27-2009, 11:16 PM   #2
Scut_Monkey
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What type of culture? A starter is a form of culture, a liquid culture. Are you asking for photos of a starter built up from a solid media like agar?

 
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Old 10-27-2009, 11:26 PM   #3
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Here is mine

[IMG][/IMG]
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Old 10-27-2009, 11:32 PM   #4
krops13
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Yes Yes from agar please.

 
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Old 10-28-2009, 12:24 AM   #5
shortyjacobs
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[noobquestion] shouldn't any starter look the same, regardless of what it comes from? [/noobquestion]

 
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Old 10-28-2009, 03:16 AM   #6
krops13
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I'm trying to propagate a starter from a agar slant. but with such low counts will it come out of suspension. And as of now I'm only getting very small trace amounts in the bottom not even enough to turn the the bottom brown. I'm trying to get my technique down.

 
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Old 10-28-2009, 03:25 AM   #7
BioBeing
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I would hazard a guess that you are trying to go from a slant to too big of a culture in one step. In the lab, I would go from a plate or slant to a 50 ml culture, then use this to inoculate a larger culture (up to about 500 ml, 1 L in a pinch).

 
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Old 10-28-2009, 03:51 AM   #8
krops13
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Last time I went straight to 400ml with little success. This time I started from 15ml then went to 800ml.

P.S. I'm not using a stir plate just the swirl as you walk by method.

 
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Old 10-28-2009, 04:33 AM   #9
Scut_Monkey
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When you are working with such small amounts of yeast a stir plate is definitely in your best interest. When I'm working with single colony forming units of yeast I start with a 10ml starter with a stir plate. I then step up to a 100ml starter than 1000ml.

 
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Old 10-28-2009, 05:55 PM   #10
krops13
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Do you have any pictures of each step built up? maybe before and after?

 
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