you can't freeze yeasties. the cell wall will burst like any other cells from ice crystals. slants are NOT freezable media... only glycerin offers the protection from ice crystal formation INSIDE the cell. the colder yeast are, the deeper the sleep.
"Back when I was in the pen, I used to beat my meat..."
"Damn ye, yellow-bellied sapsuckers; I'm a better man than all of ye milksops put together" - Blackbeard
"...I have really good sex moves; that I learned in China. I'm a sexual tiger; and feed on va-gine-a." - Jon Lajoie