Slanting yeast - Page 40 - Home Brew Forums
Register Now For Free!

Home Brew Forums > Home Brewing Beer > Fermentation & Yeast > Slanting yeast

Reply
 
Thread Tools
Old 12-28-2012, 06:22 PM   #391
FredTheNuke
Recipes 
 
Feb 2012
Charlotte, NC
Posts: 796
Liked 86 Times on 69 Posts


Quote:
Originally Posted by Roadliner View Post
I'm sure you already got this answered.. But maybe for future subscribers... Anything with a flat bottom will work. Beakers or mason jars can do starters, but I do. Prefer an Erlenmeyer flask.
1 gallon flat bottomed jugs would work great and they are cheaper than 4000ml erlenmeyer's. Finding them is a bit of a pain. I may have to buy a few from labelpeelers (4.47 each) and test them as the bottom seems fairly flat on them in the pictures.

 
Reply With Quote
Old 12-28-2012, 06:49 PM   #392
theveganbrewer
Recipes 
 
Sep 2011
Beaverton, OR
Posts: 1,929
Liked 296 Times on 209 Posts


1 gallon jugs of Carlo rosse wine also work, they are 11 or 12 bucks but you do get some booze at least.
__________________
-Retired Homebrewer

Jonathanquist Likes This 
Reply With Quote
Old 12-31-2012, 03:21 AM   #393
05m50dan
Recipes 
 
Nov 2010
Atco, NJ
Posts: 213
Liked 4 Times on 4 Posts


Thanks for the write-up! Was emailing links to my wife for months before Christmas to make sure she got me everything! Made my slants, couldn't get petri dishes, but found that wide-bottom canning jars work well too.
So here's my question...
I want to culture a bottle of Anchorage Brewing Co White IPA. It's brewed with ale yeast, then aged in wood with Brett. The beer has a strong Brett character, and I want to get the Brett out. What can I do to get the Brett, or am I going to have to culture this up as a mixed Sacro/Brett starter.

 
Reply With Quote
Old 12-31-2012, 03:57 PM   #394
inhousebrew
Recipes 
 
Aug 2012
minneapolis, minnesota
Posts: 1,465
Liked 127 Times on 114 Posts


Thanks again for this awesome write up! I slanted some American Ale and British Ale II Wyeast a few months ago and just broke out the first slant and tossed it into a cup of my canned yeast starter (which by the way is also awesome to have around). It's been sitting on there for almost 36 hours and looks nice and milky so my question which may have been already answered but I'm struggling to find it scrolling through these pages:

After that initial step should the yeast hypothetically be somewhere near the 100 billion cells that come in a normal package? And then you just step up from there to the appropriate amount? That's kind of what I was getting but I'm not sure if you came out and said it. Sorry if I'm missing something.
__________________
I hate Walder Frey...

 
Reply With Quote
Old 01-01-2013, 12:21 AM   #395
zazbnf
Senior Member
HBT_SUPPORTER.png
 
zazbnf's Avatar
Recipes 
 
Dec 2010
, Illinois
Posts: 667
Liked 100 Times on 72 Posts


Excellent information here. Any thoughts on using smaller vials? I already have some tall skinny 5ml vials, and it seems they should work the same way and would just maintain a smaller sample. Would the yeast have enough food for storage or would they not last as long?
__________________
Quote:
There are better things in life than alcohol, but alcohol makes up for not having them.
- Terry Pratchet

 
Reply With Quote
Old 01-01-2013, 08:21 AM   #396
theveganbrewer
Recipes 
 
Sep 2011
Beaverton, OR
Posts: 1,929
Liked 296 Times on 209 Posts


Quote:
Originally Posted by zazbnf View Post
Excellent information here. Any thoughts on using smaller vials? I already have some tall skinny 5ml vials, and it seems they should work the same way and would just maintain a smaller sample. Would the yeast have enough food for storage or would they not last as long?
If the slurry is pourable into those vials you'd be somewhere between 1-2 billion cells per ml. So at best I think you'd have 10 billion cells, but more than likely 5-7. You could just do more of them, add like 10 of them to your starter I guess.
__________________
-Retired Homebrewer

 
Reply With Quote
Old 01-01-2013, 04:40 PM   #397
zazbnf
Senior Member
HBT_SUPPORTER.png
 
zazbnf's Avatar
Recipes 
 
Dec 2010
, Illinois
Posts: 667
Liked 100 Times on 72 Posts


I just did some testing using water on my 5ml vials and to get a slant that doesn't cover the bottom so they won't push up, I can use 1 ml of the agar solution in each tube. I just need to buy some agar now to give them a shot.

My plan would be to build up a starter from 1 vial so I may need to add a smaller step to account for my smaller starting volume.
__________________
Quote:
There are better things in life than alcohol, but alcohol makes up for not having them.
- Terry Pratchet

 
Reply With Quote
Old 01-01-2013, 10:47 PM   #398
FredTheNuke
Recipes 
 
Feb 2012
Charlotte, NC
Posts: 796
Liked 86 Times on 69 Posts


Has anyone tried and succeeded in slanting Brett? I see no reason it won't work but wanted to ask.
__________________
Sour Fermentations In Progress

 
Reply With Quote
Old 01-04-2013, 04:42 AM   #399
peter78
Recipes 
 
Jun 2011
Washington, DC
Posts: 167
Liked 3 Times on 3 Posts


Has anyone tried the "water immersion" technique from the "yeast" book? Page 198.

 
Reply With Quote
Old 01-05-2013, 04:12 AM   #400
jason.mundy
BIAB Purist
 
jason.mundy's Avatar
Recipes 
 
Jul 2007
Ellettsville, IN
Posts: 317
Liked 19 Times on 16 Posts


Quote:
Originally Posted by peter78 View Post
Has anyone tried the "water immersion" technique from the "yeast" book? Page 198.

I haven't. I'm interested in doing it. Was a centrifuge needed to do this?
__________________
Jason

AHA - How to Brew in a Bag (BIAB): https://www.homebrewersassociation.o...w-in-a-Bag.pdf
My BIAB Kettle - Bayou Classic 1140, Fermenter - SS Brewtech Brewbucket
Beersmith Podcast - Brew in a Bag (BIAB) Down Under BeerSmith Podcast 10
Basic Brewing Podcast - BIAB, Proper Terminology
Basic Brewing Podcast - How to BIAB
Online BIAB Calculator - http://pricelessbrewing.github.io/BiabCalc/

 
Reply With Quote
Reply
Thread Tools


Similar Threads
Thread Thread Starter Forum Replies Last Post
Ethyl Alcohol Replacement for Slanting?? enderwig General Techniques 8 08-12-2014 06:09 PM
Washing yeast, starters, pre-made wort, all kinds of yeast Q's 98EXL General Techniques 15 09-06-2011 05:55 PM
Frozen Yeast Bank vs. Yeast Slanting shortyjacobs Fermentation & Yeast 11 09-24-2010 07:13 PM
WL East Coast Ale Yeast - Who has a good all grain recipe for this yeast? Griffsta Recipes/Ingredients 7 05-16-2009 09:12 PM
Made two batches, one with huge yeast slurry, and one with dry Safale-04 yeast. Jolly McStanson General Beer Discussion 4 03-07-2009 08:23 PM


Forum Jump