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Old 06-13-2011, 12:40 PM   #221
drocu
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Originally Posted by wardenwheat View Post
I may have missed this, but do you store your un-innoculated slants in the fridge too, or at room temp somewhere?
In the lab, agar plates/tubes are kept in the fridge to minimize any chance of contamination. While they should be mostly sterile and therefore fine at room temp, I'd still keep them in the fridge. Be sure that they're sealed tightly because they can dry up in the fridge too.

 
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Old 06-13-2011, 07:53 PM   #222
Saccharomyces
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It's time to re-slant my library (some of my slants are well over a year old at this point, and I have some two year old expired yeast vials I got cheap that I need to slant as well). I went to Home Depot yesterday and picked up some boards to build vial racks to incubate the old slants with sterile wort. I plan to let them incubate while I'm gone at NHC, and I'll re-slant all of my yeast strains when I get back. I'll post pics of the racks once I get them built.
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Old 07-01-2011, 01:25 AM   #223
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I tried slanting yeast for the first time on some Wyeast 3787 Trappist High Gravity. It was extremely easy and now I have five slants of an awesome yeast. If you've been thinking of trying this, do it.

 
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Old 07-02-2011, 12:06 AM   #224
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My slants looked great after setting up, but have started to turn watery after a couple weeks. I used the proportions mentioned on the first page with pharmaceutical grade agar. Any suggestions??

 
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Old 07-04-2011, 03:44 PM   #225
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Quote:
Originally Posted by wardenwheat View Post
My slants looked great after setting up, but have started to turn watery after a couple weeks. I used the proportions mentioned on the first page with pharmaceutical grade agar. Any suggestions??
Possible the mix settled before you poured it? I had that happen with one batch half went to water and had to be tossed. If they are all watery you need more agar.
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Old 07-06-2011, 12:58 AM   #226
RJSkypala
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How do these vials look for slanting?

http://cgi.ebay.com/72-pcs-Clear-4-D...b#ht_500wt_922

 
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Old 07-06-2011, 01:44 AM   #227
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Originally Posted by RJSkypala
How do these vials look for slanting?

http://cgi.ebay.com/72-pcs-Clear-4-D...b#ht_500wt_922
Question is, can the lids withstand the pressure cooker heat? Is the whole thing autoclaveable?

 
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Old 07-06-2011, 01:45 AM   #228
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I contacted the seller to ask about that.

If they are autoclaveable, are they a good size?

 
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Old 07-06-2011, 05:01 AM   #229
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Size sounds OK assuming they are autoclaveable.
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Old 07-07-2011, 06:55 AM   #230
bv805
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Whats the optimal temperature for storing the slants after incubation..I just got a mini fridge for my yeast and its sitting at about 33 F

 
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