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Old 02-16-2010, 07:10 AM   #111
displacedtexan
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Sep 2007
Posts: 17

I don't have a small scale and am having trouble measuring the 2.5 grams of agar.

I used 1/2 teaspoon and my agar hasn't set after about 12 hours.

Anybody know the volumetric equivalent of 2.5 grams of agar (e.g. 2.5 ml, 5 ml, etc).

Thanks!

 
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Old 02-16-2010, 07:12 AM   #112
mordantly
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May 2008
Westside..... CenCal - the country that'll never take away my guns or money !!!!!!!!!!!!!!!!!!!!!!!
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powder or jelly? if it's powder, ummm i can check. the jelly, can't you estimate like a cube of butter?

 
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Old 02-16-2010, 04:10 PM   #113
displacedtexan
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Sep 2007
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I have the universal beer agar powder from Midwest Brewing Supplies.

Thanks for the help!

 
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Old 02-16-2010, 06:01 PM   #114
dfohio
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Nov 2008
Columbus, OH
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My Agar is in flakes. 2.5 grams = 1Tbsp for these. Not quite sure about a powder though
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Old 02-16-2010, 07:02 PM   #115
displacedtexan
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Sep 2007
Posts: 17

Quote:
Originally Posted by dfohio View Post
My Agar is in flakes. 2.5 grams = 1Tbsp for these. Not quite sure about a powder though
Got it, thanks! I'll give it another try in a few days!

 
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Old 02-24-2010, 12:29 AM   #116
JimE
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Dec 2009
Virginia
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Quote:
Originally Posted by mordantly View Post
im sad to report, on day 5 or 6, no visible life of any kind on my three test-slants of the previously mentioned jell-o media. how long should i wait at 65-70F before i scrap the test? i think the stuff is too dry for growth.
I wouldn't use regular Jello. Try Knox or some other brand of unflavored, unsweetened gelatin. Let your wort provide all the nutrients. How did they turn out after you waited another day?

 
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Old 02-24-2010, 12:54 AM   #117
mordantly
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Westside..... CenCal - the country that'll never take away my guns or money !!!!!!!!!!!!!!!!!!!!!!!
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Quote:
Originally Posted by JimE View Post
I wouldn't use regular Jello. Try Knox or some other brand of unflavored, unsweetened gelatin. Let your wort provide all the nutrients. How did they turn out after you waited another day?
it was a complete failure:

melted at room temp
very little growth (was impregnated in media)
co2 created pockets in media
media moved around in vials
a complete pain to clean the vials
was a tricky ratio of jello/dme

pictures here

http://www.homebrewtalk.com/f163/sla...ml#post1730057

 
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Old 06-05-2010, 04:46 PM   #118
chuckjaxfl
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Feb 2010
Jacksonville, FL
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Quote:
Originally Posted by displacedtexan View Post
I don't have a small scale and am having trouble measuring the 2.5 grams of agar.

I used 1/2 teaspoon and my agar hasn't set after about 12 hours.

Anybody know the volumetric equivalent of 2.5 grams of agar (e.g. 2.5 ml, 5 ml, etc).

Thanks!
Have you considered a low-end reloading scale? Ammunition reloaders work in 0.1 grain (or 0.000228571429 ounce, or 0.006479891 gram) measurements.

Here is a low price, low tech, high precision scale. Accurate to 1/20 grain, allegedly. It will only measure up to about 1/4 ounce, though.

For a few dollars more this one is digital, and will measure up to about 3.5 ounces.

 
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Old 06-07-2010, 11:28 PM   #119
riromero
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Nov 2008
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I got one of these little scales from Harbor Freight to measure brewing water salts...

http://www.harborfreight.com/digital...ale-93543.html

I didn't have much hope that it was accurate, but I calibrated it with volumes of water and it was right on the money.

 
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Old 06-15-2010, 03:47 PM   #120
pbarning
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Apr 2010
Michigan
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Would I be correct in assuming that the pressure cooker provides a means of sterilizing both the media and vials?

I am thinking about boiling the media and funneling it into the vials. Then I'll put the filled and partially capped vials into a beaker that will be placed into a boiling water bath. I will cover the bath (pot) so as to allow the steam to make contact with the vials. This should be sufficient for sterilizing the vials, right?

-or-

Could I simply boil the media and funnel it into the vials and then place the partially capped vials into a stove for a period of time? Like a ghetto autoclave?

Bottom line is that I do not own a pressure cooker, but there should be alternative means of successfully sterilizing the media and equipment.

 
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