Yeast bank vials not freezing? - Home Brew Forums

Register Now!
Home Brew Forums > Home Brewing Beer > Fermentation & Yeast > Yeast bank vials not freezing?

Reply
 
Thread Tools
Old 08-19-2009, 08:46 PM   #1
c0bra
Recipes 
 
Jul 2009
Kansas City, KS
Posts: 52


I followed FlyGuy's instructions here: http://www.homebrewtalk.com/f13/guid...st-bank-35891/ recently and whipped up 10 vials of WY1056.

Everything seemed to go well but the next day I noticed that the contents of the vials had not frozen. I had them in a smaller cooler with ice packs as suggested, so I left the door on it open thinking it might have insulated them from the cold a bit (this is a manual-defrost freezer, so no freeze/thaw temp cycles). Several days later they're still not frozen, though some have frozen bits or slush.

What's the deal here? I tried to use about 15% glycerine (which I picked up at the LHBS) but I didn't measure and it might have been more like 25%. Could that have lowered the freezing point? I should note that this freezer has frozen several gallon jugs of water solid overnight. Will the yeast be okay, if they're at a low enough temp, or am I screwed?

TIA
__________________

Primary: Mirror Pond Clone, Moose Drool Clone

 
Reply With Quote
Old 08-19-2009, 09:01 PM   #2
bsay
 
bsay's Avatar
Recipes 
 
Jul 2008
Fort Collins
Posts: 572
Liked 3 Times on 3 Posts


How do you know they are not frozen? If you are expecting ice crystals on the inside, well that's exactly what the glycerine prevents. Try filling a spare vial 1/2 full of water, and place in the center of your yeast vials. If it freezes, then all is well.
__________________
Check out my Homebrewery Virtual Tour!

Who says you can't brew all-grain indoors?
Brewing 2.5 gal batches with a 2gal cooler MLT in my cramped apartment. Pictures coming soon!

Ask me about my Keezer/2.5 gal brew setup!

 
Reply With Quote
Old 08-19-2009, 09:15 PM   #3
c0bra
Recipes 
 
Jul 2009
Kansas City, KS
Posts: 52

Quote:
Originally Posted by bsay View Post
How do you know they are not frozen? If you are expecting ice crystals on the inside, well that's exactly what the glycerine prevents. Try filling a spare vial 1/2 full of water, and place in the center of your yeast vials. If it freezes, then all is well.
I guess I expected the slurry to still freeze, though I don't know why... I guess I didn't see it mentioned anywhere that it wouldn't.

I'm sure that a vial with water would freeze, as everything else in there freezes fine, so I guess it's OK!
__________________

Primary: Mirror Pond Clone, Moose Drool Clone

 
Reply With Quote
Old 08-20-2009, 03:10 AM   #4
passedpawn
Waste Allocation Load Lifter - Earth Class
HBT_ADMIN.png
 
passedpawn's Avatar
Recipes 
 
Apr 2009
☼ Clearwater, FL ☼
Posts: 25,865
Liked 6697 Times on 3960 Posts


Mine freeze solid. I go 15% glycerine, measured. BTW, I have re-animated 1056 (from frozen) several times without problem.

 
Reply With Quote
Old 08-20-2009, 08:18 PM   #5
rocketman768
HBT_LIFETIMESUPPORTER.png
 
rocketman768's Avatar
Recipes 
 
Feb 2008
Evanston, IL
Posts: 1,083
Liked 24 Times on 21 Posts


Whether it's solid or not does not matter. What you want is for the temperature of the yeast to be about 0F. Measure the temperature of your freezer with an infrared thermometer or one of those made to go in freezers and fridges (cheap 3-4 dollars).

 
Reply With Quote


Reply
Thread Tools


Similar Threads
Thread Thread Starter Forum Replies Last Post
Exploding Yeast Vials Brewsmith Recipes/Ingredients 14 03-14-2012 03:41 PM
Yeast vials Hammy71 General Beer Discussion 29 07-11-2011 01:38 AM
Frozen yeast bank not freezing T-Hops General Techniques 6 03-17-2010 03:20 PM
Vials for yeast bank jamesrm Equipment/Sanitation 5 02-27-2009 01:27 AM
How big are the White Lab yeast vials? Pumbaa Equipment/Sanitation 3 09-26-2006 01:14 AM


Forum Jump