Prior to last evening I had taken the yeast cake (WPL060) from a previous batch of beer and stored it in the fridge for about 2 weeks. Last night I whipped up a starter using DME and retrieved the yeast cake from the fridge.
Using a sterilized turkey baster I siphoned off the top layer and a tiny bit of the trub and placed it into my starter. This is my first time making a starter from "harvested" yeast. The others have all promptly started fermenting overnight. This morning I check the container... NADA. There were a few and I mean FEW bubbles on the surface probably left over from shaking it before going to bed.
Any suggestions on where I might have screwed up?