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Old 07-27-2009, 02:43 PM   #1
Boerderij_Kabouter
 
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So with my brewery cobbled together enough to be functional again (and much improved within
the last couple months ) I am using my budget to stream line my brewery. I have started
buying hops in bulk (hopsdirect.com) and my next step is to start a frozen yeast bank.

I have read flyguy's tutorial about a million times and have an idea of what I want to do. PLease
look though my list and read about my setup and tell me if I am forgetting anything or if you
would do things differently...

thanks

...................................

Here is my basic design for my yeast ranch:



My brewery is in the basement and I have not redone the room it is in, so dirt and dust are a
concern. The whole ranch will be within a plexi-glass "clean room" that will be completely sealed,
and vented with a sanitary air filter (the little plastic ones). Housed within the ranch will be a
fridge converted to a freezer with a LOVE controller, two stir plates, a hot plate, a cold water
bath, and my testing equipment and assorted glassware.

The reason for two stir plates is that I do yeast experiments with every batch I brew using at
least two yeast strains.

Here is my equipment list:



...........................................

I think this should be all the stuff I need.

My procedure will be to use the pressure cooker for sterilization, the hotplate for heating starter
wort, stir plates for propagation, and the vials with glycerine for freezing yeast samples.

1. Does this look like a good plan?

2. Will the plexi-glass housing be enough protection against floating nasties?

3. Would you include or do anything different?


Thanks a million!




 
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Old 07-27-2009, 02:52 PM   #2
MNBugeater
 
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Looks good. My only recommendation is that you will want more than 4 vials. I have 4 strains in my yeast bank and I have try to harvest 4 vials from each flask. So have a a total of 8 vials of each strain. When I begin a started, I put two vials in each flask.

Not saying its the right way, just that it works for me and creates good healthy starters.


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Old 07-27-2009, 02:54 PM   #3
PseudoChef
 
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I would say more vials as well. Also probably needs the sportcoat for the best effect. You will also want someway to keep condensation on the stirplates in check.

 
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Old 07-27-2009, 02:57 PM   #4

No Laminar flow-hood?

I'm disappointed in you, Justin.

I'm using 8 dram vials for my slants, believe I got a gross for around $70.


 
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Old 07-27-2009, 03:07 PM   #5
Boerderij_Kabouter
 
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Oh yeah, the vials come in packs of 12, so I figured I would have enough for 4-5 strains to start with and order more later to keep initial cost at bay.

Sport coat is a definite need.

Quote:
Originally Posted by PseudoChef View Post
You will also want someway to keep condensation on the stirplates in check.
What do you mean by this? While they are propagating or during cooling? I haven't used a stir plate for this kind of thing before, I am a complete yeast ranching noob.

 
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Old 07-27-2009, 03:10 PM   #6
Clayton
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well the plexi-glass will work kinda like a glove box. but if you want to beable to working there you need to add a laminar flow hood, there are lots of plans to build them on line
the home mushroom growers build them. but basicly all you need is a hepa filter and fan blowing air in to your box, but it needs to be centered and free of turbulance, so no shrouds or cover on the filter, the eddys in the curent can mix in contams.

as for slants i use these

http://cgi.ebay.com/20-Vacutainer-47...3286.m20.l1116

i also got some autoclavable plastic caps for mason jars and added red port from a VACUTAINER, that way i just take a suringe and put yeast from the VACUTAINER and in to the mason jar that has autoclaved wort in side totaly steral transfer in to steral wort.
i will take some pics of the lids tonight.
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Old 07-27-2009, 03:11 PM   #7

I'd strongly consider adding Parafilm to your list of supplies, particularly if you are planning on testing viability of your frozen samples by streaking to an agar plate.

Don't buy agar media from a supply company, the price is ridiculous. It's easily found at health food stores (vegetarian gelatin substitute).

 
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Old 07-27-2009, 03:40 PM   #8
Clayton
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Quote:
Originally Posted by flyangler18 View Post
I'd strongly consider adding Parafilm to your list of supplies, particularly if you are planning on testing viability of your frozen samples by streaking to an agar plate.

Don't buy agar media from a supply company, the price is ridiculous. It's easily found at health food stores (vegetarian gelatin substitute).
yeah +1 Parafilm

get you a few glass petry dished too , thay are costly but the plastic ones
cant be autoclaved and you will waste some many becase thay come in sleaves of 20 and you wont need but maybe four to isolate and clean up the dirtyest ones from bottle beer you like that has yeast in it.

here is some good pricing on agar agar
Bulkfoods.com Agar Powder 5 pounds Fast Online ordering with $5 Shipping on $75+ order.
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Old 07-27-2009, 03:54 PM   #9
PseudoChef
 
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Nevermind, I didn't read your description well enough. I thought the whole hood was refrigerated. I'm a dummy.

 
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Old 07-27-2009, 04:26 PM   #10
Boerderij_Kabouter
 
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What is this parafilm you speak of?

And for the agar plates, do you streak them when you pitch the starter yeast and check under a microscope to double check you have the sacc. yeast you wanted? Like I said, this is all new to me.

Thanks for the help guys!!!



 
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