Did you wash the yeast or anything like that? I'm sure it would be just fine to throw into your batch, but I would honestly still make a starter anytime I'm reusing yeast. I'm sure the grain bill is quite expensive on your barley wine. Hate to see it go to waste by a bad batch of yeast. Normally if I'm pitching on top of another yeast cake I'd do it very soon after racking off of it.
"Brewers enjoy working to make beer as much as drinking beer instead of working."