This thread is awesome. I have an experiment on going and will post photos soon, but I had a question.
I have a few strains of wild yeast that metabolize bromocresol green - so they are white/opaque when grown on BCG, yet they are sensitive to cyclohexamide at 10-20 mg/L.
From what I've read, Brett should be resistant to cyclohexamide. Any ideas to what these yeast might be. They are clearly budding yeast.
Doing some more searching, it seems possible that they might be Candida? That would suck as all my isolates have the same phenotype. One more edit. I don't think many Candida can tolerate cycloheximide.
Another update. I actually got one clone after 3 days at 30 degrees C that grew on cyclohexamide (last picture). It's looks no different than (colony morphology or cells) the cyc negative yeast.
I grew small cultures (10 mL in DME with a small bit of yeast extract) and neither of the cultures have much flavor at all? Do I need larger/longer grows? Yes, I might need to grow the yeast on cycloheximide for 7-10 days.
Ok, here are some photos of two wild yeast clones isolated from different sources.
What do you guys think? I think they might be Brett.
cyclohexamide resistant Clone 2