The beer in question is Great Divide's Colette. I've done a simple culture of the yeast and am decently happy with the results, but according to the label they use a blend of four different strains, one apparently being a wild strain as per someone on this forum directly asking Great Divide. I would like to have a separate culture of each strain so I can hopefully come up with a unique blend that I like the best.
I do have a basic knowledge of microbiology and the SOPs of growing cell cultures and streak plates and such, but let me reiterate that it is very basic. I was wondering if anyone on here had any experience of doing this, I tried searching but didn't really come up with anything useful. What I was planning on doing so far based on my knowledge was:
1) Flame neck and cap of beer.
2) Open up bottle of beer, pour into glass (for later consumption of course).
3) Flame opening of bottle, swirl up sediment.
4) Dip inoculation loop into dregs, proceed to streak onto agar plate.
5) Repeat for multiple bottles and plates.
After this is where I'm kinda lost. How will I know the difference between the different strains? Don't most yeast strains look extremely similar when grown on agar? I only have experience growing E. Coli, and only the DH5-α strain at that, so I'm only used to seeing milky white colonies.
I do have a basic knowledge of microbiology and the SOPs of growing cell cultures and streak plates and such, but let me reiterate that it is very basic. I was wondering if anyone on here had any experience of doing this, I tried searching but didn't really come up with anything useful. What I was planning on doing so far based on my knowledge was:
1) Flame neck and cap of beer.
2) Open up bottle of beer, pour into glass (for later consumption of course).
3) Flame opening of bottle, swirl up sediment.
4) Dip inoculation loop into dregs, proceed to streak onto agar plate.
5) Repeat for multiple bottles and plates.
After this is where I'm kinda lost. How will I know the difference between the different strains? Don't most yeast strains look extremely similar when grown on agar? I only have experience growing E. Coli, and only the DH5-α strain at that, so I'm only used to seeing milky white colonies.