I get worried about infections mostly when I'm twinking with slants and plates. This took a lot of worry out of it, at least for me. I'm kinda anal....
DIY yeast glove box w/pics
- Thread starter sudbuster
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homebrewer_99
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Sorry, but you're in the "UBER-ANAL" section now...
HIM_Tattoos
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Sorry, but you're in the "UBER-ANAL" section now...
"UBER-ANAL" ?? Doe's that mean "a real arsehole?
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Wow. Just, wow. I've never seen anything like that in the homebrew setting. Jamil Z. will be jealous (if he doesn't have one already).
Jamil does't worry about contamination.. He's a "beer god"
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Scut_Monkey
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That's pretty creative and I love the toilet flanges. However, how can you culture yeast with sterile technique using that? I understand it isolates dust particles but how are you maintaing sterility of your equipment in there by not being able to flame?
Scut_Monkey
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ventilation is only needed for the alcohol burner, which is also not needed , but makes me feel better. there is a couple of these on the back. Pic later..
http://www.austinhomebrew.com/product_info.php?products_id=2289
Scut_Monkey
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So you are using an alcohol lamp in it? Just curious.
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The box is sanitized by spaying inside with a disinfectant on all surfaces and gloves 30 min before use. All equiptment is sterilized beforehand in a pressure cooker at 15lb for 30 min, then unwrapped inside the box. I do use a flame for the needle and loupe, although even as anal as I am, it is probably overkill..
jds
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You must have a good-natured cat. I wouldn't try it unless the gloves were armored.
Bokonon
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Well if you are working with multiple cultures I don't see that the flame is overkill unless you have separate inoculation loops for every culture you are doing.
On short notice tonight I made 5 plates, stepped up 2 starters from 10ml to 100ml, and made 2 2L starters. All around my little alcohol burner on my clothes washer.
As cool as I think it would be to have something like this I think it would just increase the time it takes to do everything.
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Maybe it takes me longer to use the box, I don't know. But I'm retired and like to mess with it anyway. What's more, I know I've done all that can be done to prevent contaminating my yeast strains...
Rushis
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If you aren't filtering the air in the box how is it anymore sterile than your countertop? Manipulating your samples inside the glovebag, as you mentioned, is slower that doing it with unencumbered hands. The key for a success in microbiology is quick and accurate procedures that minimize the opportunity for contaminination. For delicate samples a laminar flow hood is the proper tool.
A glovebag would be a much simpler DIY solution.
Just look at how happy this biology student is
Large clear plastic bag, kitchen gloves, duct tape, and an air compressor hooked to a hepa filter to pressurize the bag.
Just some thoughts, nice work on the box.
A glovebag would be a much simpler DIY solution.
Just look at how happy this biology student is
Large clear plastic bag, kitchen gloves, duct tape, and an air compressor hooked to a hepa filter to pressurize the bag.
Just some thoughts, nice work on the box.
That thing is BAD!!!!! I love it! I want one.
jtrainer
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Are you talking about the glove box or the biology student er I mean the glove bag?
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If you aren't filtering the air in the box how is it anymore sterile than your countertop? Manipulating your samples inside the glovebag, as you mentioned, is slower that doing it with unencumbered hands. The key for a success in microbiology is quick and accurate procedures that minimize the opportunity for contaminination. For delicate samples a laminar flow hood is the proper tool.
A glovebag would be a much simpler DIY solution.
Just look at how happy this biology student is
Large clear plastic bag, kitchen gloves, duct tape, and an air compressor hooked to a hepa filter to pressurize the bag.
Just some thoughts, nice work on the box.
The box is filtered through a hepa filter as linked above. I used the plastic bag idea for a while, but it was a PITA. Have ypu priced a laminar flow hood?
In the words of my favorite actor/ philosopher Clint "opinions are like ********,......." and "get off my lawn". Thanks for the fresh input and cool pix, especially the bio student...
Oh,and" minimizing the opportunity of contamination "was the impetus for this project.
This box is over $2000. I've got $18 invested in mine.
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Thanks, WM. I'm doing all my starters. dishes and slants in it now. we'll see how it turns out...
Ah you beat me to the punch with the glovebag comment. You can make them (or get them made) by taking a heat sealer and melts the plastic around the edge. As mentioned above you would want to use hepa filters and maintain a positive pressure on the bag. When I used them we had sleeves that came out of the glove bag/box that were permanently attached, but the gloves were inverted, taped and pushed into the sleeve. That way you can repair or change out gloves if it becomes contaminated or torn. You don't want to use the same gloves twice until they've been deconned. We would do smoke tests on the bag to make sure that they were sealed properly too. Your glovebox does look awesome though!
Edit: I just wanted to clarify that it wasn't actual smoke that we used. It was this little tube that you'd crack. I didn't want anyone to read that and think that I was lighting a fire inside a glove bag...
Edit: I just wanted to clarify that it wasn't actual smoke that we used. It was this little tube that you'd crack. I didn't want anyone to read that and think that I was lighting a fire inside a glove bag...
mordantly
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A glovebag would be a much simpler DIY solution.
Just look at how happy this biology student is
she looks a tad innnerbrated. well erm. innebreated.
yeqmaster
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your insane!
mordantly
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thank you.
Rushis
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Glovebag's are a huge PITA, I agree. I spent 2 months restoring a 30 year old inert atmosphere box just to avoid working with that constantly hissing and pseudo effective monstrosity.
Laminar flow hood runs $2-3k installed for a new low end model. That is assuming you have the duct work in place to exhaust it properly and the bells/whistles to make it a functional workspace (plumbing, vacuum pumps, 220V outlet). I wasn't meaning to imply that it is a practical option for the homebrewer, but I am of the firm mindset that the pros know what they are doing and can provide inspiration.
I saw your link to the AHS hepa filters. But I didn't see where on your rig you had a blower hooked up to recirculate air through them. What sort of pump do you have moving the air through filters? Do you purge the box atmosphere or recirculate through your hepa filters after you've moved items in through the antechamber?
I'm really curious to see what you've hooked for an air handler in this beast.
The box!!! That thing rocks!:rockin:
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WM, you have the coolest insulation on your brew pots that i have ever seen..:rockin:
Thanks Sudbuster, they sure were fun to build. The design was the hardest part. Still don't like the HLT as much as the MT though.
Calichusetts
Well-Known Member
6 years....
That's why I said I must sub for posterity's sake. I like the idea if I were ever in need of a DIY glove box. If not, I may just buy one depending on finances and need.
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