I don't think it matters b/c I've had kegs that I force carbed and everything, but could tell they were still green (not ready to drink) I didn't take it off the gas. Just didn't drink it for a while and it aged fine. Obviously aging w/o the Co2 works we all do that all the time so I sort of have experimental data to say it doesn't matter, but my suggestion is don't pressurize the keg until you at least think it is ready. Why waste the CO2?