Stepping up a starter

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I failed with the search to answer a question that's probably been answered before.

Should I be oxygenating the new wort before adding it to my starter when stepping it up?:confused:

thx.
 
Yes. The original wort will have had its O2 level depleted. If you want to increase the cell count by stepping up, the new wort will need to have O2 introduced so that yeast will continue to increase their cell count.
 
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