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Search: Posts Made By: bluedubbed
Forum: Recipes/Ingredients 08-16-2013, 05:24 PM
Replies: 6
Views: 2,317
Posted By bluedubbed
Just a bump. Anyone try this and have any input? ...

Just a bump. Anyone try this and have any input?
Thanks.
Forum: Lambic & Wild Brewing 01-25-2013, 03:33 PM
Replies: 946
Views: 237,664
Posted By bluedubbed
I'm in NA - Utah. We live at about 8000 ft and...

I'm in NA - Utah. We live at about 8000 ft and most of my samplings have been in the mountains between 7-10,000 ft. Probably has something to do with what yeast I'm getting.
I'm not sure about...
Forum: Lambic & Wild Brewing 01-24-2013, 08:48 PM
Replies: 946
Views: 237,664
Posted By bluedubbed
Huh, that's interesting. I putatively...

Huh, that's interesting.

I putatively identify Sacc (and maybe others?) vs Brett first by collecting everything on an agar plate with either no drugs or just antibiotic to inhibit bacterial...
Forum: Lambic & Wild Brewing 01-24-2013, 02:55 PM
Replies: 946
Views: 237,664
Posted By bluedubbed
One of the 3 wild Brett I have growing just...

One of the 3 wild Brett I have growing just formed a pellicle.

These are the yeast that formed this pellicle.
Forum: Lambic & Wild Brewing 01-17-2013, 09:40 PM
Replies: 946
Views: 237,664
Posted By bluedubbed
Hey Dinnerstick. The plates I use are not...

Hey Dinnerstick.

The plates I use are not that much different than what you have. I use WLN (recipe here - http://www.condalab.com/pdf/1086.pdf) supplemented with 10 ug/ml chx (first few...
Forum: Lambic & Wild Brewing 01-14-2013, 09:14 PM
Replies: 946
Views: 237,664
Posted By bluedubbed
I've captured wild Sacc, but I've only been...

I've captured wild Sacc, but I've only been interested in Brett so I've never kept any strains or tested them out. Based on my wild collections, I seem to get more Sacc (and possibly other...
Forum: Lambic & Wild Brewing 01-03-2013, 12:58 PM
Replies: 946
Views: 237,664
Posted By bluedubbed
I don't have any solid evidence to definitively...

I don't have any solid evidence to definitively answer your question, but I've been able to capture yeast as low as 35-40 F. I haven't tried anything lower.
Let us know how things turn out.
Forum: Lambic & Wild Brewing 12-27-2012, 01:05 AM
Replies: 946
Views: 237,664
Posted By bluedubbed
dinnerstick - very neat. I'll have to give that...

dinnerstick - very neat. I'll have to give that a try some time.

Another question for the thread:

I finally got around to tasting the 3 wild brett fermentations that I have as gallon...
Forum: Lambic & Wild Brewing 12-19-2012, 03:35 PM
Replies: 946
Views: 237,664
Posted By bluedubbed
dinnerstick - Where did you get your bugs for...

dinnerstick - Where did you get your bugs for your wild cider? Just off the fruit?
Cool to see Kloeckera apiculata.
Forum: Lambic & Wild Brewing 12-10-2012, 11:09 PM
Replies: 946
Views: 237,664
Posted By bluedubbed
Here are some long overdue photos. These are all...

Here are some long overdue photos. These are all wild yeast (and other stuff) that were captured by rubbing different things on the plates. Juniper berries, pine cones, Russian sage flowers,...
Forum: Lambic & Wild Brewing 11-28-2012, 10:44 PM
Replies: 946
Views: 237,664
Posted By bluedubbed
I'm not sure how this relates to others...

I'm not sure how this relates to others experiences using wort to harvest yeast and bacteria, but I collected wild yeast using agar plates. I did everything from leaving the plates outside and...
Forum: Lambic & Wild Brewing 11-09-2012, 08:07 PM
Replies: 946
Views: 237,664
Posted By bluedubbed
The photos are at 64X. They should be pure since...

The photos are at 64X. They should be pure since the grows were from single colonies after a few rounds of selection. I'll take a few photos of the plates, but surprisingly, colony morphology...
Forum: Lambic & Wild Brewing 11-09-2012, 05:40 PM
Replies: 946
Views: 237,664
Posted By bluedubbed
Got 2 new isolates that smell super funky when...

Got 2 new isolates that smell super funky when grown in WLN media. I'm going to do a few mini batches this weekend with 3 different isolates and see what flavors/aromas these guys produce with...
Forum: Lambic & Wild Brewing 10-24-2012, 08:19 PM
Replies: 946
Views: 237,664
Posted By bluedubbed
Another question. I have over 10 different...

Another question.
I have over 10 different pure Brett cultures (or at least I think so) isolated at various times and places over many hundreds of miles. While I bet most are the same, I thought...
Forum: Lambic & Wild Brewing 10-24-2012, 02:50 PM
Replies: 946
Views: 237,664
Posted By bluedubbed
dinnerstick- Not sure based on morphology, but I...

dinnerstick- Not sure based on morphology, but I found that plates with chloramphenicol (25ug/ml) were really helpful in getting rid of bacterial contamination and cycloheximinde (10-20 ug/ml) were...
Forum: Lambic & Wild Brewing 10-22-2012, 10:01 PM
Replies: 946
Views: 237,664
Posted By bluedubbed
jaapie- Thanks for the link to your post. I...

jaapie- Thanks for the link to your post.
I isolated 10 more cycloheximide resistant clones in the mountains this weekend.
Time to start seeing what kind (hopefully different) flavors they...
Forum: Lambic & Wild Brewing 10-22-2012, 03:22 PM
Replies: 946
Views: 237,664
Posted By bluedubbed
Yeah, there are some simple PCR assays that will...

Yeah, there are some simple PCR assays that will distinguish Brett from others. As posted above and this paper as well - http://www.ncbi.nlm.nih.gov/pubmed?term=Tessonni%C3%A8re%20h

Also, this lab...
Forum: Lambic & Wild Brewing 10-18-2012, 09:53 PM
Replies: 946
Views: 237,664
Posted By bluedubbed
This thread is awesome. I have an experiment on...

This thread is awesome. I have an experiment on going and will post photos soon, but I had a question.
I have a few strains of wild yeast that metabolize bromocresol green - so they are...
Forum: Fermentation & Yeast 11-25-2011, 12:27 AM
Replies: 4
Views: 451
Posted By bluedubbed
Thanks for the replies. I was essentially trying...

Thanks for the replies. I was essentially trying to keep potential contamination down by using selective media. I just want to store and reuse a few strains I use a lot.

daksin - Thanks. ...
Forum: Fermentation & Yeast 11-23-2011, 09:29 PM
Replies: 4
Views: 451
Posted By bluedubbed
Growing Wyeast Strains in Selective Media

Does anyone know what selective media Wyeast Labs uses to grow their strains? For example, maybe the American Ale 1056 strain is URA-. I'm sure not all are the same and it's a fairly easy...
Forum: Sponsor Giveaways 06-01-2011, 05:54 PM
Replies: 4,439
Views: 111,281
Posted By bluedubbed
i want one

i want one
Forum: Lambic & Wild Brewing 01-28-2011, 08:11 PM
Replies: 43
Views: 4,748
Posted By bluedubbed
Thanks. I just found that thread!

Thanks. I just found that thread!
Forum: Lambic & Wild Brewing 01-28-2011, 05:27 PM
Replies: 43
Views: 4,748
Posted By bluedubbed
Just a follow up to jalgayer's questions. How...

Just a follow up to jalgayer's questions. How much fruit, maltodextrin, DME or honey would you add at a time? If adding maltodextrin or DME do you dissolve it in water first?

Kind of an...
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