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schultan · 02-20-2008, 11:20 PM

I don't think that a GC will be required for that procedure. From the looks of it, you can get by with an old Spec20. These can be actually quite affordable, and are very easy to use.

mrkristofo · 02-21-2008, 05:15 AM

Quote:

Originally Posted by schultan

I don't think that a GC will be required for that procedure. From the looks of it, you can get by with an old Spec20. These can be actually quite affordable, and are very easy to use.

The quarts cuvettes needed for UV, however, are not that affordable.

dpt222 · 02-23-2008, 02:11 AM

Quote:

The quarts cuvettes needed for UV, however, are not that affordable.

I've seen quartz cuvettes for less than $50 a piece. You only need one, but several are nice to have.

This may be a nice experiment for a biochemistry lab to do...or maybe trade a bio/chem Prof at a university his spectrophotometric analysis for some homebrew. Trust me, most science professors enjoy beer.

avidhomebrewer · 02-23-2008, 04:00 AM

Yeah, you need sophisticated lab equipment (a spectrophotometer, amongst other things). Solvents such as toluene and hexanes can be rather nasty to play around with in quantity; you can get nasty headaches from toluene. If you know what types of hops you are growing, you can estimate the AA for what is currently on the market. You probably won't be that far off.

mrkristofo · 02-23-2008, 04:05 AM

Quote:

Originally Posted by dpt222

This may be a nice experiment for a biochemistry lab to do...or maybe trade a bio/chem Prof at a university his spectrophotometric analysis for some homebrew. Trust me, most science professors enjoy beer.

I was serious about my offer, actually (in fact I kinda wanna play with it...pleeeaaassseeeee). I have access to everything required...just gotta run it by the PI. I could even do HPLC to get specific % of Humulone, Cohumulone, *****one, and the beta acids. Could, but probably wouldn't. Simple organic extraction plus a scan on the UV/Vis would be a piece of cake.