The Great Bottle Opener Giveaway

Home Brew Forums > Home Brewing Beer > DIY Projects > Yeast ranch - Is this all I need

Reply
 
LinkBack Thread Tools
Old 07-27-2009, 02:43 PM   #1
Boerderij_Kabouter
Feedback Score: 0 reviews
 
Boerderij_Kabouter's Avatar
Recipes 
 
Join Date: Dec 2007
Location: Oconomowoc, Wisconsin
Posts: 8,461
Liked 115 Times on 100 Posts
Likes Given: 11

Default Yeast ranch - Development and Build

So with my brewery cobbled together enough to be functional again (and much improved within
the last couple months ) I am using my budget to stream line my brewery. I have started
buying hops in bulk (hopsdirect.com) and my next step is to start a frozen yeast bank.

I have read flyguy's tutorial about a million times and have an idea of what I want to do. PLease
look though my list and read about my setup and tell me if I am forgetting anything or if you
would do things differently...

thanks

...................................

Here is my basic design for my yeast ranch:



My brewery is in the basement and I have not redone the room it is in, so dirt and dust are a
concern. The whole ranch will be within a plexi-glass "clean room" that will be completely sealed,
and vented with a sanitary air filter (the little plastic ones). Housed within the ranch will be a
fridge converted to a freezer with a LOVE controller, two stir plates, a hot plate, a cold water
bath, and my testing equipment and assorted glassware.

The reason for two stir plates is that I do yeast experiments with every batch I brew using at
least two yeast strains.

Here is my equipment list:



...........................................

I think this should be all the stuff I need.

My procedure will be to use the pressure cooker for sterilization, the hotplate for heating starter
wort, stir plates for propagation, and the vials with glycerine for freezing yeast samples.

1. Does this look like a good plan?

2. Will the plexi-glass housing be enough protection against floating nasties?

3. Would you include or do anything different?


Thanks a million!


Last edited by Boerderij_Kabouter; 01-04-2010 at 08:06 PM.
Boerderij_Kabouter is offline
 
Reply With Quote Quick reply to this message
Old 07-27-2009, 02:52 PM   #2
MNBugeater
Feedback Score: 0 reviews
 
MNBugeater's Avatar
Recipes 
 
Join Date: Jan 2006
Location: Minneapolis, MN
Posts: 979
Liked 7 Times on 5 Posts

Default

Looks good. My only recommendation is that you will want more than 4 vials. I have 4 strains in my yeast bank and I have try to harvest 4 vials from each flask. So have a a total of 8 vials of each strain. When I begin a started, I put two vials in each flask.

Not saying its the right way, just that it works for me and creates good healthy starters.

__________________

On Tap: Blonde Ale, Apfelwein, Cinnamon Apfelwein, Amber Ale, Irish Red
Bottled: Porter, Wheat, Barleywine, Cranberry Apfelwein, Amber Ale
Buy Bugeater Brewery Gear

MNBugeater is offline
 
Reply With Quote Quick reply to this message
Old 07-27-2009, 02:54 PM   #3
PseudoChef
Feedback Score: 0 reviews
 
PseudoChef's Avatar
Recipes 
 
Join Date: Apr 2007
Location: West Chicago 'Burbs, IL
Posts: 3,418
Liked 102 Times on 75 Posts
Likes Given: 39

Default

I would say more vials as well. Also probably needs the sportcoat for the best effect. You will also want someway to keep condensation on the stirplates in check.

__________________
PseudoChef is offline
 
Reply With Quote Quick reply to this message
Old 07-27-2009, 02:57 PM   #4
flyangler18
Feedback Score: 0 reviews
Recipes 
 
Join Date: Jun 2008
Location: Hanover, PA
Posts: 5,679
Liked 25 Times on 25 Posts
Likes Given: 5

Default

No Laminar flow-hood?

I'm disappointed in you, Justin.

I'm using 8 dram vials for my slants, believe I got a gross for around $70.

__________________

Last edited by flyangler18; 07-27-2009 at 03:06 PM.
flyangler18 is offline
 
Reply With Quote Quick reply to this message
Old 07-27-2009, 03:07 PM   #5
Boerderij_Kabouter
Feedback Score: 0 reviews
 
Boerderij_Kabouter's Avatar
Recipes 
 
Join Date: Dec 2007
Location: Oconomowoc, Wisconsin
Posts: 8,461
Liked 115 Times on 100 Posts
Likes Given: 11

Default

Oh yeah, the vials come in packs of 12, so I figured I would have enough for 4-5 strains to start with and order more later to keep initial cost at bay.

Sport coat is a definite need.

Quote:
Originally Posted by PseudoChef View Post
You will also want someway to keep condensation on the stirplates in check.
What do you mean by this? While they are propagating or during cooling? I haven't used a stir plate for this kind of thing before, I am a complete yeast ranching noob.
Boerderij_Kabouter is offline
 
Reply With Quote Quick reply to this message
Old 07-27-2009, 03:10 PM   #6
Clayton
Feedback Score: 0 reviews
Recipes 
 
Join Date: Apr 2008
Location: NE Oklahoma
Posts: 1,493
Liked 18 Times on 16 Posts
Likes Given: 19

Default

well the plexi-glass will work kinda like a glove box. but if you want to beable to working there you need to add a laminar flow hood, there are lots of plans to build them on line
the home mushroom growers build them. but basicly all you need is a hepa filter and fan blowing air in to your box, but it needs to be centered and free of turbulance, so no shrouds or cover on the filter, the eddys in the curent can mix in contams.

as for slants i use these

http://cgi.ebay.com/20-Vacutainer-47...3286.m20.l1116

i also got some autoclavable plastic caps for mason jars and added red port from a VACUTAINER, that way i just take a suringe and put yeast from the VACUTAINER and in to the mason jar that has autoclaved wort in side totaly steral transfer in to steral wort.
i will take some pics of the lids tonight.

__________________
Once, during Prohibition, I was forced to live for days on nothing but food and water. ~ W.C. Fields

Last edited by Clayton; 07-27-2009 at 03:13 PM. Reason: fixed ebay link had wrong link
Clayton is offline
 
Reply With Quote Quick reply to this message
Old 07-27-2009, 03:11 PM   #7
flyangler18
Feedback Score: 0 reviews
Recipes 
 
Join Date: Jun 2008
Location: Hanover, PA
Posts: 5,679
Liked 25 Times on 25 Posts
Likes Given: 5

Default

I'd strongly consider adding Parafilm to your list of supplies, particularly if you are planning on testing viability of your frozen samples by streaking to an agar plate.

Don't buy agar media from a supply company, the price is ridiculous. It's easily found at health food stores (vegetarian gelatin substitute).

__________________
flyangler18 is offline
 
Reply With Quote Quick reply to this message
Old 07-27-2009, 03:40 PM   #8
Clayton
Feedback Score: 0 reviews
Recipes 
 
Join Date: Apr 2008
Location: NE Oklahoma
Posts: 1,493
Liked 18 Times on 16 Posts
Likes Given: 19

Default

Quote:
Originally Posted by flyangler18 View Post
I'd strongly consider adding Parafilm to your list of supplies, particularly if you are planning on testing viability of your frozen samples by streaking to an agar plate.

Don't buy agar media from a supply company, the price is ridiculous. It's easily found at health food stores (vegetarian gelatin substitute).
yeah +1 Parafilm

get you a few glass petry dished too , thay are costly but the plastic ones
cant be autoclaved and you will waste some many becase thay come in sleaves of 20 and you wont need but maybe four to isolate and clean up the dirtyest ones from bottle beer you like that has yeast in it.

here is some good pricing on agar agar
Bulkfoods.com Agar Powder 5 pounds Fast Online ordering with $5 Shipping on $75+ order.
__________________
Once, during Prohibition, I was forced to live for days on nothing but food and water. ~ W.C. Fields
Clayton is offline
 
Reply With Quote Quick reply to this message
Old 07-27-2009, 03:54 PM   #9
PseudoChef
Feedback Score: 0 reviews
 
PseudoChef's Avatar
Recipes 
 
Join Date: Apr 2007
Location: West Chicago 'Burbs, IL
Posts: 3,418
Liked 102 Times on 75 Posts
Likes Given: 39

Default

Nevermind, I didn't read your description well enough. I thought the whole hood was refrigerated. I'm a dummy.

__________________
PseudoChef is offline
 
Reply With Quote Quick reply to this message
Old 07-27-2009, 04:26 PM   #10
Boerderij_Kabouter
Feedback Score: 0 reviews
 
Boerderij_Kabouter's Avatar
Recipes 
 
Join Date: Dec 2007
Location: Oconomowoc, Wisconsin
Posts: 8,461
Liked 115 Times on 100 Posts
Likes Given: 11

Default

What is this parafilm you speak of?

And for the agar plates, do you streak them when you pitch the starter yeast and check under a microscope to double check you have the sacc. yeast you wanted? Like I said, this is all new to me.

Thanks for the help guys!!!

__________________
2P-Twent-E Simple Brew DITCHES 1 DITCHES 2 Fermentolagertaporator side-by-side ferm Twelve Hopostles Labels
Boerderij_Kabouter is offline
 
Reply With Quote Quick reply to this message
Reply


Quick Reply
Message:
Options
Thread Tools


Similar Threads
Thread Thread Starter Forum Replies Last Post
Bleu Cheese Vs Ranch GregR General Chit Chat 88 10-30-2011 06:29 AM
Liquid Yeast--Do Kits in Sequence or Split Yeast Pack? osagedr Beginners Beer Brewing Forum 10 02-27-2010 02:24 PM
WL East Coast Ale Yeast - Who has a good all grain recipe for this yeast? Griffsta Recipes/Ingredients 7 05-16-2009 09:12 PM
Southwest style hamburger with chipotle ranch fries and irish coffee cupcakes Laurel Cooking & Pairing 10 02-15-2009 05:16 PM
Flying Bee Ranch - Great Honey Source! BrewFrick Mead Forum 4 03-29-2008 12:24 AM