Every time you wash it you're losing some yeast, it just happens. To some extent it doesn't matter when you're making starters, you just have to do more steps or bigger starters.
Primary: Wise Jefe (Hefeweizen)
Secondary: What sorcery is this?
Future: Alchemist Heady Topper Clone, Yooper's DFH 60 Minute Clone, Session Bitter