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Home Brew Forums > Home Brewing Beer > Beginners Beer Brewing Forum > Yeast Reconsititution Fail?
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Old 01-22-2009, 12:27 AM   #1
stephelton
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Default Yeast Reconsititution Fail?

When racking my last Irish Red Ale to its secondary fermenter last November, I attempted to take a sample of the yeast for reconstitution later. On monday, I attempted to make a starter out of it. So far, I don't see any visible signs of yeast activity, though there does appear to be more 'sludge' at the bottom of my 1/2 gallon jug. I was hoping to do a brew later tonight (after Lost but I'm having second thoughts about the viability of the yeast.

Is the lack of visible activity a sure sign (after 2 days) that I failed somewhere?

I'll go into detail about what exactly I did if anyone cares to hear it

Thanks!

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Old 01-22-2009, 12:32 AM   #2
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Starters can ferment pretty quick. Check your gravity and see where you're at. Assuming you aimed for around 1.04, you ought to be around 1.01X or so.

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Old 01-22-2009, 12:35 AM   #3
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good call..! thanks!

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Old 01-22-2009, 12:35 AM   #4
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Starters can go very quickly. I've had a couple that worked, but I missed activity entirely.

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Old 01-22-2009, 12:50 AM   #5
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yeah, huge fail. sg is where it was.

So... i followed the directions here:

http://www.homebrewtalk.com/f13/yeas...strated-41768/

And am wondering... if the water I used was about 45 degrees F, and the yeast was at about 70, would my pouring that water have shocked the yeast? I don't know what else I could have done wrong... I used sugar for the yeast to munch on in my starter, (and although that may not be optimal) I'm sure that's fine. The SG was a lot higher than I calculated (about 1.06)...

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Old 01-22-2009, 12:59 AM   #6
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Sugar is definitely not good to make starters with for other reasons, but I"m thinking the 25 degree difference definitely could have shocked them.

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Old 01-22-2009, 01:21 AM   #7
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That's what I'm thinking. I meant to pick up some light DME for starters last time I was at the LHS, forgot to. Guess I'll be going back there for yeast tomorrow anyway, I'll grab some for next time. Bummer..! I've got a bunch of other yeast I treated in the same manner, probably not any good either.

Thanks for the tips.

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Old 01-22-2009, 03:08 PM   #8
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Are you running a stirplate? I have found that a stirplate (like the cheap/free ghetto ones some of us fab around here) are immensely helpful for building up wort from a tiny amount of live cells.

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Old 01-22-2009, 03:29 PM   #9
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Quote:
more 'sludge'
Sounds good. A starter is growing more yeast, NOT fermenting. Very little CO2 is produced during the growth stage.
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Old 01-22-2009, 04:20 PM   #10
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Quote:
Originally Posted by david_42 View Post
Sounds good. A starter is growing more yeast, NOT fermenting. Very little CO2 is produced during the growth stage.
Ah yes, I should go review my Papazian again...

fratermus, you're just ...I guess east of me? I'm in 75080!
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