Electric Brewing Supply 30A BCS Giveaway!


Home Brew Forums > Home Brewing Beer > Beginners Beer Brewing Forum > Yeast culturing
Reply
 
LinkBack Thread Tools
Old 02-21-2009, 03:29 AM   #1
SGT-RIEL
Feedback Score: 0 reviews
Recipes 
 
Join Date: Feb 2009
Posts: 41
Default Yeast culturing

I've read the post on yeast washing, but what I'm looking for is a tutorial on splitting up a live culture into pitchable amounts for long term use. Any help or advice would be grateful. Looking to stay busy in between brews.

__________________
SGT-RIEL is offline
 
Reply With Quote Quick reply to this message
Old 02-21-2009, 04:02 AM   #2
fratermus
Feedback Score: 0 reviews
Recipes 
 
Join Date: Feb 2008
Location: 75081
Posts: 1,189
Liked 2 Times on 1 Posts

Default

I'm not sure I know what you're shooting for. Normally culturing refers to tiny amounts, certainly not suitable for pitching.

If you mean culturing on agar media, here is a solid (but rambling) overview of culturing.

Here is my crappy thumbnail sketch of yeast conservation methods.

fratermus is offline
 
Reply With Quote Quick reply to this message
Old 02-21-2009, 05:12 AM   #3
SGT-RIEL
Feedback Score: 0 reviews
Recipes 
 
Join Date: Feb 2009
Posts: 41
Default

What I'm getting at, is buying a pitchable vial of yeast and culturing it further into more pitchable vials for use 6 months to a year from now.

__________________
SGT-RIEL is offline
 
Reply With Quote Quick reply to this message
Old 02-21-2009, 11:50 AM   #4
KerryD
Feedback Score: 0 reviews
Recipes 
 
Join Date: Dec 2008
Location: Katy TX
Posts: 156
Liked 1 Times on 1 Posts

Default

I think you would look for a wiki on building a really large starter and then just split that up.

__________________
Kemp's Ridley Brewing Company

Primary I: Barley Wine Divine #6 attempt
Primary II: Saint Arnold's Elissa Clone
3 Gal BB: Ed Wort's Apfelwein
Next up: .....
Bottled: Wing and a Prayer IPA, AHS Blue Moon, Centennial Blond, Yoopers Dogfish 60 Clone, Texas Wheat, SN Celebration Ale clone, Ed's Haus Pale Ale, 6 variations of Apfelwein
KerryD is offline
 
Reply With Quote Quick reply to this message
Old 02-21-2009, 01:33 PM   #5
planenut
HBT_SUPPORTER.png
Feedback Score: 0 reviews
 
planenut's Avatar
Recipes 
 
Join Date: Aug 2008
Location: Hanahan, SC (North Charleston)
Posts: 536
Liked 2 Times on 2 Posts

Default

I think this is what you are looking for...

http://www.homebrewtalk.com/f13/guid...st-bank-35891/

__________________
planenut is offline
 
Reply With Quote Quick reply to this message
Old 02-21-2009, 03:06 PM   #6
fratermus
Feedback Score: 0 reviews
Recipes 
 
Join Date: Feb 2008
Location: 75081
Posts: 1,189
Liked 2 Times on 1 Posts

Default

Quote:
Originally Posted by SGT-RIEL View Post
What I'm getting at, is buying a pitchable vial of yeast and culturing it further into more pitchable vials for use 6 months to a year from now.
That is not commonly done so I don't think there will be much literature on it.

Normally pure cultures are saved for reuse by culturing them on media, storing them cooled (or frozen), then stepping the back up to pitchable size.

If you wanted to go for it, here are my first thoughts on a possible procedure:

1. Sterilize (in oven, autoclave, or pressure canner) everything but the yeast: flask, foil, funnels, whatever. I'd have the starter+nutrient in the flask already and sterilze in situ. Allow to cool.*

2. Pitch yeast into flask of starter, preferably on a stirplate.

3. Allow it to run long enough that you are convinced there are no fermentables left.

4. Sterilize some bottles and funnel, sanitize caps. Based on my unscientific testing the White Labs vials also survive pressure sterilization and will still seal, although some of the flanges warp. I think we are going to want more volume than a vial, though, unless you can achieve massive starter density in step 3.

5. pour the maxxed out starter into the bottles, cap**

6. store in fridge (preferably inside a bin in case there are contaminants or residual sugars and they BTFU) until use.***

7. I'd still pitch these into starters instead of into the primary, but then again I generally do not consider vials/packs directly pitchable anyhow.

Frater Mus
* I love pressure canners for this. I recently bought a taller canner that allows me to sterilize even my biggest flasks standing up with the foil caps in place.
** yes, I know that capping will be controversial. But I think that if the OP is storing a half-dozen bottles of "pitchable" starter in the fridge caps are more practical.
*** Mr. Safety would suggest wearing eye and hand protection in case of overpressured
fratermus is offline
 
Reply With Quote Quick reply to this message
Old 02-21-2009, 03:19 PM   #7
SGT-RIEL
Feedback Score: 0 reviews
Recipes 
 
Join Date: Feb 2009
Posts: 41
Default

Quote:
Originally Posted by planenut View Post
I think this is what you are looking for...

http://www.homebrewtalk.com/f13/guid...st-bank-35891/

Awesome that's exactly what i was looking for. Thanks guys for your help.
__________________
SGT-RIEL is offline
 
Reply With Quote Quick reply to this message
Old 02-22-2009, 03:45 AM   #8
SGT-RIEL
Feedback Score: 0 reviews
Recipes 
 
Join Date: Feb 2009
Posts: 41
Default

Anyone know a place that will sell this stuff in a kit?

__________________
SGT-RIEL is offline
 
Reply With Quote Quick reply to this message
Old 02-22-2009, 12:21 PM   #9
fratermus
Feedback Score: 0 reviews
Recipes 
 
Join Date: Feb 2008
Location: 75081
Posts: 1,189
Liked 2 Times on 1 Posts

Default

AFAIK no one sells freezing kits. I bought most of my lab gear off eBay and basicsciencesupplies.com.

Keep in mind that frozen yeast cultures are not pitchable in any normal sense of the word.

__________________
Adequate Mousetrap brewhouse

Batch history | yeast slants | Suspending yeast
fratermus is offline
 
Reply With Quote Quick reply to this message
Old 02-22-2009, 08:48 PM   #10
SGT-RIEL
Feedback Score: 0 reviews
Recipes 
 
Join Date: Feb 2009
Posts: 41
Default

would it hurt anything if i used the plastic corning vials my yeast comes in for my frozen yeast bank?

__________________
SGT-RIEL is offline
 
Reply With Quote Quick reply to this message
Reply



Quick Reply
Message:
Options
Thread Tools


Similar Threads
Thread Thread Starter Forum Replies Last Post
Is culturing lager yeast from a bottle harder than ale yeast? hexmonkey Fermentation & Yeast 2 08-31-2009 05:23 PM
Culturing Yeast Corjyn Beginners Beer Brewing Forum 8 08-06-2009 08:36 PM
Culturing Yeast Drifter General Beer Discussion 4 01-30-2009 12:07 PM
Yeast Culturing eurc51 Beginners Beer Brewing Forum 1 05-14-2008 12:54 PM
Yeast culturing grnich Beginners Beer Brewing Forum 9 09-10-2006 04:12 AM