Jason: I have a few comments/questions.
Quote:
Originally Posted by jason.mundy
I then dipped a metal skewer 1/4" into the olive oil and then dipped the skewer in my Erlenmeyer flask prior to the starter boil.
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Prior to the boil...I wonder if boiling can damage the oil molecules. Some others on the forum mentioned adding the oil after boiling...but then what to do about sterilizing it if you didn't boil?
Quote:
Originally Posted by jason.mundy
Once cooled, I added my WLP002 yeast and put the flask on a stir plate for 48 hours.
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Do you use an airlock or just foil? Since you don't need O2 in your starter, I'm curious if you are aerating the starter plus adding oil. Have you considered a procedure like this?
1. Grow up an aerated starter (e.g. 2L on stir plate w/ foil)
2. Chill and decant off most of the supernatant beer
3. Add the oil (drop or whatever) and resuspend the yeast to mix
4. Let sit for a few few hours while brewing so the oil is taken up by the yeast
5. Pitch into 5 gal non-aerated wort
This way the wort is not aerated, the yeast starter had all the O2 it needed during it's growth phase, and the yeast got a fresh supply of oil to help it deal with growing in the 5 gal of wort. The Grady Hull oil thesis mentioned that adding olive oil doesn't supply the sterols needed for yeast reproduction, just the fatty acids. One of Grady's suggestions was a combo of an aerated starter plus olive oil. See thesis quote below (in italics):
In order to achieve a healthy, vigorous fermentation yeast requires both sterols and fatty acids. In this study only oleic acid [i.e. fatty acids] was provided to the yeast. ... Oxygenating yeast at the beginning of yeast storage may provide the yeast with the sterols necessary for proper fermentation at a time when the yeast is healthy enough to withstand some metabolic activity (Smart 2000). This, together with olive oil addition, may provide the combination of sterols and fatty acids necessary for a proper fermentation. The addition of olive oil and sterols could also be combined with a reduction in wort aeration to achieve a vigorous fermentation with reduced oxygen exposure.