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Home Brew Forums > Home Brewing Beer > Beginners Beer Brewing Forum > Frozen Yeast Starter not Fermenting
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Old 08-07-2011, 07:19 AM   #1
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Default Frozen Yeast Starter not Fermenting

So I saved some yeast about 3 mos ago and they have been in my deep freeze since then. I pulled of of the first fermentatiion on my German Lager. I dilluted the yeast with a 10ml Glycerine 10 ml water 40-50 ml yeast slurry. I have now gotten around to brewing again, thanks to a 70mi move for work. I pitched 2 vials into a 1qt starter of around 1.050 (DME simple beer) after two days the yeast don't show much activity and using a refrac I don't notice much change.

My questions follow:

Are my yeast active and just slow? if so how long should I wait?

Should I of done a smaller starter and then stepped it up? Should I step it up now?

I would like to brew this week with the yeast if possible, but do have a smack pack and another beer I could do instead. I was just hoping to recreate that lager I made 3 mos ago. (It was gone in 4 days!)

Thanks in advance

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Old 08-07-2011, 04:45 PM   #2
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I think, I may be wrong, but I think that freezing yeast is a no no. I think it kills yeast to freeze it.

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Old 08-07-2011, 06:56 PM   #3
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i think you just got to give it more time. that's a lot of wort for that small amount of yeast. next time make yourself a small starter about 250ml let that ferment out and step it up. i would also make yourself a stir plate if you don't already have one. they are cheap to make and really help out when it comes to yeast!!

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Old 08-08-2011, 03:17 AM   #4
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Well by no means am i going to throw it....Ill just brew something else for now and keep waiting. I need to get a stirplate just havn't yet. Thanks for the advice

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Old 08-10-2011, 10:21 PM   #5
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Quote:
Originally Posted by jtupper View Post
Well by no means am i going to throw it....Ill just brew something else for now and keep waiting. I need to get a stirplate just havn't yet. Thanks for the advice
So what happened with that yeast did it ever take off??
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Old 08-11-2011, 01:42 AM   #6
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you could have just pitched a new vile of yeast or some 05

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Old 08-11-2011, 04:11 PM   #7
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It hasn't finished yet, I believe its just being sluggish so im still waiting. In the meantime Im going to do another starter....This time much smaller and step it up

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Old 08-11-2011, 05:03 PM   #8
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Let me know how it goes since i have a ton of frozen yeast and i haven't used any yet. Am still building my yeast farm.

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Old 08-11-2011, 05:40 PM   #9
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freezing any biological thing kills it... except for some very rare exceptions (some kind of frog exists that basically has anti-freeze in its blood - but then, it's not freezing, is it). Ice crystals form, expand, and like a million little daggers, perforates cell membranes. If you have a workaround for this, the Nobel Prize in cryogenics is awaiting you. If any fermentation is happening, then the 1 out of a billion yeast cells that miraculously survived is going to need some help.. like from a vial of fresh yeast.

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Old 08-11-2011, 06:01 PM   #10
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Thats why I always freeze yeast using Glycerine. Op knows what he is doing.

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