Originally Posted by Joe_in_CT
I saw something about adding glycerin to the yeast before freezing. I haven't started to wash my yeast yet, but freezing might be a good way to go.
What do you think happened with your keeve attempt? Could you have missed period when the chapeau brun had risen?
I've only just started researching how to save a yeast strain so there is still a lot to learn. Just putting the yeast in the fridge seems like it would not make the yeast completely dormant as the extra half gallon I had definitely fermented in there. I haven't yet come across any info on adding glycerin but maybe that's just what is needed to protect the yeast for long term storage in such temps. Also considering attempting to develop this strain into something long term.
The keeve failed due to user error. I didn't realize the clarity of the juice I used was due to it having been filtered until too late
. Luckily I bought a second kit of PME and CaCl from Chris to save on shipping.