So I've read through all of the threads in this subforum, trying to digest as much info as I can. I know that there were some threads on using bananas in the mash but IIRC they all referenced using RIPE bananas. Has anyone tried using green bananas?
I ask because I read the following abstract from Journal of Food Science
Volume 46, Issue 5, pages 14001403, September 1981:
Following subcellular fractionation and centrifugation, the amylase activity was located in the cytosol fraction of banana fruit. Over 80% of the observed activity (1520 units per mg of protein) was attributed to α-amylase. The activity of β-amylase was tenfold lower and starch phosphorylase activity was low (17 μg inorganic phosphorus released per mg protein per 24 hr.). The activity of amylase in crude preparations was stimulated 40% by calcium ions. The amylase preparation, which was very stable at 4°C, hydrolyzed soluble potato starch and banana starch at similar rates. Maximum activity was observed between pH 67. The energy of activation of hydrolysis was 9.74 Kcal/mole. Amylase was quite active up to 62°C but rapidly lost activity above this temperature. There was an approximate twofold increase in amylase during the initial phase of ripening.
I'm just wondering if the full article describes how they get the "crude preperation" of enzymes, and if we could make it feasable.
And it seems there may be some actual number for DP of bananas in the actual article. Does anyone have access to the Wiley Online Library that can pull this down?Here is the link to the article.