I also have been recently washing my yeast. I've collected from my last three brews and I am getting ready to reuse a strain for the first time. However, I am not sure how much yeast is necessary to get a 1L starter going. I've attached a picture of 3 mason jars of WLP001 I collected from a brew. The jar on the right clearly has plenty of yeast and a little trub, but I do not know if the middle and right jars have enough yeast on their own. Please note that the right two jars have no trub. For those of you that have experience, what is a good rule of thumb when looking at volumes of yeast to use as a starter? I know mileage and personal results may vary, but I am looking for some guidance. Thanks!