Spike Brewing 12.5 Conical Fermenter Giveaway!

Home Brew Forums > Home Brewing Beer > Fermentation & Yeast > Yeast Washing

Reply
 
LinkBack Thread Tools
Old 09-11-2012, 05:33 AM   #1
mlager
Feedback Score: 0 reviews
Recipes 
 
Join Date: Mar 2011
Location: Scottsdale, AZ
Posts: 43
Default Yeast Washing

I acquired these nifty plastic vials, they are similar to the vials that the White Labs yeast comes in. I see a lot of people harvest their yeast and store them in jars. I'm thinking about harvesting and storing in these vials. If I were to do so, would I expect that one of these vials filled with my harvested yeast would be similar to the cell count of something like a white labs vial? I would always be doing a starter, so I'm wondering if it would make sense for me to use more than 1 of these vials when doing so. Any advice or information would be great!

image-1518916184.jpg

__________________
mlager is offline
 
Reply With Quote Quick reply to this message
Old 09-11-2012, 05:36 AM   #2
troyh
HBT_SUPPORTER.png
Feedback Score: 0 reviews
 
troyh's Avatar
Recipes 
 
Join Date: Jul 2012
Posts: 168
Liked 27 Times on 20 Posts

Default

I use 40ml vials for washed yeast and make starters with them and they've been fine. I think White Labs vials are 35ml so I don't see why it'd be different.

__________________
troyh is offline
 
Reply With Quote Quick reply to this message
Old 09-11-2012, 05:42 AM   #3
mlager
Feedback Score: 0 reviews
Recipes 
 
Join Date: Mar 2011
Location: Scottsdale, AZ
Posts: 43
Default

Nice, good to hear. I think I'm going to give it a shot. These vials just seem easier to store in the fridge. And honestly, make me feel like a scientist ha but that's just a secondary benefit.

__________________
mlager is offline
 
Reply With Quote Quick reply to this message
Old 09-11-2012, 05:50 AM   #4
bratrules
Feedback Score: 0 reviews
Recipes 
 
Join Date: Oct 2010
Location: socal
Posts: 455
Liked 18 Times on 10 Posts
Likes Given: 6

Default

Those are nice for keeping frozen yeast. Where did you get them?

__________________

Alcohol may be man's worst enemy, but the bible says love your enemy. - Frank Sinatra

bratrules is offline
 
Reply With Quote Quick reply to this message
Old 09-11-2012, 05:56 AM   #5
mlager
Feedback Score: 0 reviews
Recipes 
 
Join Date: Mar 2011
Location: Scottsdale, AZ
Posts: 43
Default

http://www.sciencebob.com/store/pre-150.php

Apparently these are what two liter soda bottles start out as... They seem super durable and have seal nice and tight.
__________________
mlager is offline
 
Reply With Quote Quick reply to this message
Old 09-11-2012, 06:02 AM   #6
bratrules
Feedback Score: 0 reviews
Recipes 
 
Join Date: Oct 2010
Location: socal
Posts: 455
Liked 18 Times on 10 Posts
Likes Given: 6

Default

Cool thanks for the link. They will work great for a frozen yeast bank!!

__________________

Alcohol may be man's worst enemy, but the bible says love your enemy. - Frank Sinatra

bratrules is offline
 
Reply With Quote Quick reply to this message
Old 09-11-2012, 06:15 AM   #7
mlager
Feedback Score: 0 reviews
Recipes 
 
Join Date: Mar 2011
Location: Scottsdale, AZ
Posts: 43
Default

I would have always thought freezing yeast would kill me, I was planning on throwing me into the fridge... Is there anything special that must be done to freeze me and bring e back to life other than using a starter? Do they last a lot longer frozen?

__________________
mlager is offline
 
Reply With Quote Quick reply to this message
Old 09-11-2012, 06:24 AM   #8
bratrules
Feedback Score: 0 reviews
Recipes 
 
Join Date: Oct 2010
Location: socal
Posts: 455
Liked 18 Times on 10 Posts
Likes Given: 6

Default

Check this out http://www.homebrewtalk.com/f13/guide-making-frozen-yeast-bank-35891/ but I also wash yeast to!! Combining both really saves you some cash.

__________________

Alcohol may be man's worst enemy, but the bible says love your enemy. - Frank Sinatra

bratrules is offline
 
Reply With Quote Quick reply to this message
Old 09-11-2012, 01:54 PM   #9
ajf
Senior Member
HBT_LIFETIMESUPPORTER.png
Feedback Score: 0 reviews
 
ajf's Avatar
Recipes 
 
Join Date: Oct 2005
Location: Long Island
Posts: 4,643
Liked 99 Times on 93 Posts
Likes Given: 39

Default

Yeast from White Labs is prepared under laboratory conditions. It will not lose viability as fast as washed yeast, and is 100% yeast, whereas your yeast will contain a certain amount of trub. In other words, your vials will contain much less viable yeast than an equivalent sized vial of yeast from White Labs. I use jars to store mine, which allows me to make a starter with just one jar. You will probably need to use more than one vial, or make a multi-step starter.
I can recommend http://www.amazon.com/Yeast-Practical-Fermentation-Brewing-Elements/dp/0937381969 as a source of some great information on the subject.

-a.

__________________

There are only 10 types of people in this world. Those that understand binary, and those that don't.

ajf is offline
 
Reply With Quote Quick reply to this message
Old 09-11-2012, 02:54 PM   #10
troyh
HBT_SUPPORTER.png
Feedback Score: 0 reviews
 
troyh's Avatar
Recipes 
 
Join Date: Jul 2012
Posts: 168
Liked 27 Times on 20 Posts

Default

According to Jamil and George Fix, the slurry in washed yeast is at least 75% yeast, probably more, assuming you washed it properly. And a White Labs vial isn't full of yeast either. There's probably 20-30ml of yeast slurry in a White Labs vial. They claim 100B cells total, so that's 3.3-5B cells per ml. Washed yeast should have 2.5-3.75B cells per ml (probably more). So if you have 27-40ml of washed yeast slurry in a vial, you have about the equivalent of a White Labs vial or a Wyeast pack.

__________________
troyh is offline
 
Reply With Quote Quick reply to this message
Reply


Quick Reply
Message:
Options
Thread Tools