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Old 01-24-2012, 03:10 PM   #1
Spica66
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Default Yeast slant media recipe

In the Slanting Yeast sticky there are two conflicting recipes for the media.

On page one:
400mL of water
35g of DME
2.5g agar agar

On page eight:
500mL of water
7.5 grams of agar
35 grams of DME
5 grams of the nutrient


I would like to know if anyone out there has a recipe that never fails them. Something you have been been able to repeat with success. I have powdered agar agar, so my ideal recipe would use that. Thank you in advance for sharing!

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Old 01-24-2012, 04:35 PM   #2
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I wasted alot of time and DME trying to get the powdered agar to work with no success. As soon as my agar agar came in from amazon I made up the media using pretty close to the same recipe as on page eight minus the nutrient.

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Old 01-24-2012, 04:43 PM   #3
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Quote:
Originally Posted by Westel View Post
I wasted alot of time and DME trying to get the powdered agar to work with no success. As soon as my agar agar came in from amazon I made up the media using pretty close to the same recipe as on page eight minus the nutrient.
If powdered agar agar didn't work, what type did?

Here is what I have:

http://www.asiansupermarket365.com/T...p/htbaayej.htm

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Old 01-24-2012, 05:17 PM   #4
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I used the recipe on page one and used powdered agar. Worked well, but I did have some fluid in the slant, although I am not concerned as it is sterile. The agar slants are still good to go a month later. I used some this morning, and it is easy to poke the agar media with a inoculation loop.

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Old 01-24-2012, 05:20 PM   #5
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I used the first one with agar from morebeer and they fell apart, used a recipe similar to the second and I have to boil the slants to get the media out after I make a starter.

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Old 01-24-2012, 05:23 PM   #6
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Quote:
Originally Posted by sapperxl View Post
I used the first one with agar from morebeer and they fell apart, used a recipe similar to the second and I have to boil the slants to get the media out after I make a starter.
Similar? Would you mind posting the actual recipe?
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Old 01-24-2012, 05:24 PM   #7
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Here's what I have now http://www.amazon.com/Roland-Strips-...429397&sr=8-10

The stuff I couldn't get to work was from midwest not sure why but I could never get it to set up.

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Old 01-25-2012, 02:03 PM   #8
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Quote:
Originally Posted by Spica66
Similar? Would you mind posting the actual recipe?
Looked up my notes and it's the exact same recipe as the second, I just doubled it so I could fill more slants, I make about 200 a time since they store almost indefinitely.
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Old 01-25-2012, 04:15 PM   #9
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Quote:
Originally Posted by sapperxl View Post
Looked up my notes and it's the exact same recipe as the second, I just doubled it so I could fill more slants, I make about 200 a time since they store almost indefinitely.
Next time I need yeast I'm skipping MW and just calling sapper

My recipe I used was 250 ml h20
3.4g agar agar
17.5g DME
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Old 01-25-2012, 04:19 PM   #10
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Default I have decided on a recipe.

Taking into account everything I could read on the internet and the responses received from the fine people on this forum, I am going to use the following recipe for attempt #1:

1% = 1g/100ml

7.0% DME
1.5% Agar-agar
1.0% Yeast Nutrient


200ml Tap Water
14g DME
3g Powdered Agar-agar
2g Yeast Nutrient
Red food coloring
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