Ss Brewing Technologies Giveaway!

Home Brew Forums > Home Brewing Beer > Fermentation & Yeast > Yeast propagation best practices
Reply
 
LinkBack Thread Tools
Old 10-15-2009, 11:58 AM   #1
cscade
HBT_LIFETIMESUPPORTER.png
Feedback Score: 0 reviews
 
cscade's Avatar
Recipes 
 
Join Date: Feb 2009
Location: Wooster, Ohio
Posts: 456
Liked 15 Times on 12 Posts
Likes Given: 1

Default Yeast propagation best practices

I picked up a couple expired vials of White Labs WLP026 on clearance at the LHBS, but I'm not planning to use them for months, at least. Really I bought them because I'm very interested in yeast culturing and I'd like to see if I can practice with this strain.

It was suggested in this thread that I just leave the vials alone, and culture them shortly before I plan to actually use them.

But already the yeast in the vials has a mottled look to it, some light creamy, some darker creamy colored. No browns or blacks yet. It is about 5 months old.

I understand that if I make a starter, wash, and re-store, I'm running the risk of contamination. However, I am very, very clean and it seems that this would be better for the colony than just letting it continue to age.

I use erlenmeyer flasks and a stir plate for my starters. I do not have an oxygenation setup. Will propagating multiple starters without forced oxygenation make weak yeast? Thoughts otherwise?

__________________

Seeker Brewing Co.
est. 2008
Build Thread - Blog
cscade is offline
 
Reply With Quote Quick reply to this message
Old 10-15-2009, 12:09 PM   #2
elmetal
Feedback Score: 0 reviews
Recipes 
 
Join Date: Sep 2007
Location: Pembroke Pines, FL
Posts: 1,040
Liked 1 Times on 1 Posts

Default

I would make the slants from the actual vial, not from a washed starter.

__________________
Bottled: Imperial Stout[/SIZE]
elmetal is offline
 
Reply With Quote Quick reply to this message
Old 10-15-2009, 12:10 PM   #3
elmetal
Feedback Score: 0 reviews
Recipes 
 
Join Date: Sep 2007
Location: Pembroke Pines, FL
Posts: 1,040
Liked 1 Times on 1 Posts

Default

unless you're saving the wash from a starter and not making a slant. then I don't really know as I don't do that kind of thing. I make slants.

__________________
Bottled: Imperial Stout[/SIZE]
elmetal is offline
 
Reply With Quote Quick reply to this message
Old 10-15-2009, 12:20 PM   #4
cscade
HBT_LIFETIMESUPPORTER.png
Feedback Score: 0 reviews
 
cscade's Avatar
Recipes 
 
Join Date: Feb 2009
Location: Wooster, Ohio
Posts: 456
Liked 15 Times on 12 Posts
Likes Given: 1

Default

I'm planning to make slants, but I'm not there yet. It will be a while before I have the right equipment. So for now, washing the starter and storing in jars was the plan.

Also, I'd like to make a lot of sterile canned 1.040 wort ahead of time to make my starters easier. I've been making starters with DME, but would there be anything wrong with simply using enough 2-row to hit 1.040, with a little yeast nutrient added to the boil? I'd then sterilize/can this in a pressure canner and just crack open the jars and use them on starter day. Since 1qt is very close to 1L, this would be really really easy.

__________________

Seeker Brewing Co.
est. 2008
Build Thread - Blog
cscade is offline
 
Reply With Quote Quick reply to this message
Old 10-15-2009, 12:31 PM   #5
elmetal
Feedback Score: 0 reviews
Recipes 
 
Join Date: Sep 2007
Location: Pembroke Pines, FL
Posts: 1,040
Liked 1 Times on 1 Posts

Default

that sounds like a great idea and would definitely work.

I need to do that! I have a ton of mason jars that would be perfect!

__________________
Bottled: Imperial Stout[/SIZE]
elmetal is offline
 
Reply With Quote Quick reply to this message
Old 10-16-2009, 01:29 AM   #6
sudbuster
This ain't my first rodeo....
HBT_LIFETIMESUPPORTER.png
Feedback Score: 0 reviews
 
sudbuster's Avatar
Recipes 
 
Join Date: Jul 2007
Location: Western Arkansas
Posts: 4,068
Liked 273 Times on 215 Posts
Likes Given: 95

Default

Quote:
Originally Posted by cscade View Post
I'm planning to make slants, but I'm not there yet. It will be a while before I have the right equipment. So for now, washing the starter and storing in jars was the plan.

Also, I'd like to make a lot of sterile canned 1.040 wort ahead of time to make my starters easier. I've been making starters with DME, but would there be anything wrong with simply using enough 2-row to hit 1.040, with a little yeast nutrient added to the boil? I'd then sterilize/can this in a pressure canner and just crack open the jars and use them on starter day. Since 1qt is very close to 1L, this would be really really easy.
Canning enough starter wort can be a Real PITA if you have to build up a large starter. Lately i've been brewing 7 gal batches of 1.040 wort with nutrients and hops, then freezing it in one gallon bags. So far, it's worked well.
__________________
sudbuster is offline
 
Reply With Quote Quick reply to this message
Old 10-16-2009, 01:35 AM   #7
elmetal
Feedback Score: 0 reviews
Recipes 
 
Join Date: Sep 2007
Location: Pembroke Pines, FL
Posts: 1,040
Liked 1 Times on 1 Posts

Default

are you brewing with the hops for storage purposes?

__________________
Bottled: Imperial Stout[/SIZE]
elmetal is offline
 
Reply With Quote Quick reply to this message
Old 10-16-2009, 03:41 AM   #8
sudbuster
This ain't my first rodeo....
HBT_LIFETIMESUPPORTER.png
Feedback Score: 0 reviews
 
sudbuster's Avatar
Recipes 
 
Join Date: Jul 2007
Location: Western Arkansas
Posts: 4,068
Liked 273 Times on 215 Posts
Likes Given: 95

Default

Quote:
Originally Posted by elmetal View Post
are you brewing with the hops for storage purposes?
Not really, only to replicate the actual fermintation environment a bit more closely ( 1 ounce/ 7 gal ).
__________________
sudbuster is offline
 
Reply With Quote Quick reply to this message
Old 10-16-2009, 04:33 AM   #9
elmetal
Feedback Score: 0 reviews
Recipes 
 
Join Date: Sep 2007
Location: Pembroke Pines, FL
Posts: 1,040
Liked 1 Times on 1 Posts

Default

meh. if I had enough hops. wait... I have 4 pounds.

either way, I dont hop my starters... works well.

__________________
Bottled: Imperial Stout[/SIZE]
elmetal is offline
 
Reply With Quote Quick reply to this message
Old 10-16-2009, 12:32 PM   #10
cscade
HBT_LIFETIMESUPPORTER.png
Feedback Score: 0 reviews
 
cscade's Avatar
Recipes 
 
Join Date: Feb 2009
Location: Wooster, Ohio
Posts: 456
Liked 15 Times on 12 Posts
Likes Given: 1

Default

There seems to be a 50/50 split on this one. Although I certainly understand the potential merits of matching propagation environment to wort environment, it's still not "the same". Often times the sugar concentration is higher in real wort (many of us brew > 1.040 beers) and I would think that would have more of a "shock" impact on the yeast than presence of hops.

The reason I don't hop my starters is because the hop oils are known to coat the yeast cells and hamper their ability to reproduce. Although I suspect the effect is minimal, my goal in a starter is to grow out as many cells as possible.

I don't think there's a "right answer" though, so have at it!

__________________

Seeker Brewing Co.
est. 2008
Build Thread - Blog
cscade is offline
 
Reply With Quote Quick reply to this message
Reply



Quick Reply
Message:
Options
Thread Tools


Similar Threads
Thread Thread Starter Forum Replies Last Post
Yeast Propagation ipscman Brew Science 13 02-04-2011 04:32 PM
Yeast Propagation Stir Plate shane7676 Equipment/Sanitation 5 10-17-2009 02:32 PM
Yeast Propagation lyacovett General Techniques 12 07-25-2009 12:32 AM
Yeast Propagation Questions Faulkulees Beginners Beer Brewing Forum 6 07-01-2009 08:09 PM
Lager Starters - Best practices ?? stoutaholic General Techniques 8 02-20-2009 07:45 PM