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Old 12-07-2010, 03:51 PM   #21
Ichthy
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I don't have an HBS close by, well not one worth patronizing, so prolonging the inevitable is good for me.

I may give this is shot and will report back if I do.

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Old 01-22-2014, 05:11 AM   #22
ldave
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Quote:
Originally Posted by Ichthy View Post
There was a link posted above from the whitelabs website. They recommend using phosphoric acid, so starsan should be fine.

If I'm interpreting their article correctly, you should build a starter from wash yeast and once it's finished add enough acid to reach a pH of 2 (the no-rinse concentration of star san). The article then says to continually stir the culture.

So, I'm thinking of making a starter with washed yeast. Chilling and decanting once the starter is done. Then add enough premixed starsan to thin the slurry. Place the the starter back on the stir plate to mix for the recommended 60-90 min.

Does anyone see an issue with acid washing in this manner??
While Starsan is phosphoric acid based (50%), it also contains dodecylbenzenesulfonic acid (15%). This component is in the family known as 'sulfonimides', and, thus, is a powerful anti-life agent. There is much more to its killing power than simply low pH, unlike phosphoric acid. Using it to yeast wash would be an interesting experiment. I suspect a near 100% kill rate of everything in the flask. Have you done this experiment?

BTW, the reason I know alot about Starsan is because I'm sensitive to the sulfonimide component of it. It makes me extremely sun sensitive (major itchy rash). I had a pretty miserable summer the year I started using it until I figured out that I had to wear dairyman's gloves when working with it (0% exposure).
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Old 01-23-2014, 12:16 AM   #23
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Read through the sticky on Slanting Yeast. There is some good info in that thread on how to propagate a heathy strain of yeast, propagating form a starter, etc. Also the last few chapters of the White/Jamil yeast book talk about this as well.

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