Originally Posted by Gunfighter04
When I do a 1L yeast starter and let it set for a bit and the yeasties start to multiply, just before I pitch it into the wert, what if I were to return the starting volume to the vial and refrigerate. How long could I continue to do this? Would I be able to get a year or so out of a single vial?
I do that, but I store it in 8 ozs mason jars. I let it settle out and replace the wort/beer in the jar with distilled water. It will keep for a year in the fridge. Sanitation is extremely important.
I harvest yeast cakes and brew several beers from the initial starter, but don't want every beer to be from that yeast, so eventually I stop using it. When I decide I want to use that yeast again, I make a new starter from the original saved yeast, and then save a little more.
It can take 5 years to get through 5 generations.