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-   -   Wild Yeast Capture Attempt 1 (http://www.homebrewtalk.com/f163/wild-yeast-capture-attempt-1-a-403353/)

theonetrueruss 04-07-2013 04:39 PM

4 Attachment(s)

The first 2 are from last night, the next 2 are from this morning.


theonetrueruss 04-07-2013 04:39 PM

4 Attachment(s)

The first 2 are from last night, the next 2 are from this morning.


theonetrueruss 04-07-2013 04:39 PM

4 Attachment(s)

The first 2 are from last night, the next 2 are from this morning.


theonetrueruss 04-07-2013 04:39 PM

4 Attachment(s)

The first 2 are from last night, the next 2 are from this morning.


theonetrueruss 04-12-2013 03:02 AM

1 Attachment(s)

Ok, today there was a very small amount of bubbles on top of the wort. I put a sanitized stir bar in, switched to foil and put it on my stir plate.


theonetrueruss 04-16-2013 04:38 AM

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so here is an image of what it looks like now... I think that looks an awful lot like fermentation.


theonetrueruss 05-07-2013 03:59 AM

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ok, so I finally got some plates that I could pull a few colonies off of. Just did that. Used 1 isolated colony per slant. Hopefully they grow in the slants then I will do sample 3G batch split between three 1G jugs each with one of the yeasts.

The picture is a but blury but you can see the yeast in the plates. One of the plates I was still too heavy handed with and didn't get enough separation but there was plenty on the other two that I could use.


MattHollingsworth 05-07-2013 10:36 PM

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I have a few wild tests going, including the one in the picture here. Nice pellicle on this one. BUT, I used juice in this to drop the pH. I used 30% grapefruit juice in the wort for the initial capture of this stuff, then stepped it up with 250 ml of wort to 25 ml of lemon juice. No mold, just a nice looking pellicle, IMHO. Because of the juice, though, I can't properly judge the character. Smells like citrus!

So, that being the case, I want to chill this, decant and make a normal starter and see what I have here. With that in mind, what's the deal with that pellicle? Should I try to decant that off and just pitch what's settled? Or is it composed of top cropping yeast?

For the record, I have 4 sets of tests going. 2 used juice in the initial wort and both have what seems to be a better result, ie no mold and a nice fermentation. The 2 without juice both have white and green mold growing on them. Collection times and wort were different though, so it's not a proper control.

Base wort here at the start was Black IPA wort taken from a batch I brewed. Just siphoned off a bit after chilling. So, heavy hops here could have helped in addition to the juice.


MattHollingsworth 05-07-2013 10:42 PM

1 Attachment(s)

For comparison, here's one of the other tests with the mold growing on it.

These are both wort in jars, not petri dishes.

Base wort at the start was leftover from an APA I brewed. Some hops but not a lot.


MattHollingsworth 05-08-2013 06:42 AM

1 Attachment(s)
Quote:
Originally Posted by theonetrueruss View Post
my guess is that pellicle is not yeast based... but wtf do I know? not sure best route here as I am just learning about wild yeast myself... interested to know where the pellicle stuff goes though.
Maybe it's not yeast. But there's yeast in there. I saw it fermenting in a classical way for yeast for the first day, bubbles rising, krauesen, etc. Second morning, it looked like a krauesen covered with white powder. Lots of large bubble shapes covered in powder. It looked a lot like some pictures if you run a Google images search for "brettanomyces fermentation:

brettanomyces fermentation

A bit after that, it looked like this picture below. This is once the bubbling had settled down a bit. Still powdery, but only small bubbles.

After that, it looked like the first picture I posted before. Then after that, it developed a "ropy" look on the surface. Same powdery look, but then it had some ropy looking tendrils spider webbing the surface. From reading Wild Brews, on page 114, sounds like it could possibly be pedio.

Quote:
"Pedioccocus produces a layer on top of the wort resembling long elastic threads best described as ropy and a hazy viscosity, a bit like oil."
It seems to me to be a mixed culture. I don't think it's pure or anything. But from the pics I've seen of Brett fermentations, it looked like that at certain points. Only used Brett once myself and used it in primary and it didn't even get a pellicle.

Anyway, I intend to decant this and make a fresh starter, so was wondering if anyone knows what the pellicle is composed of, even if it's a mixed culture. Not precisely what it is, if its mixed, but if it's composed of stuff I want to keep or stuff I want to toss or it doesn't matter. At the moment, I intend to chill for 3-4 days, pour off everything except what's settled, pellicle included and pitch into a fresh starter.


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