Happy HolidaySs Giveaway - Winners Re-Re-Re-Re-Drawn - 24 hours to Claim!

Get your HBT Growlers, Shirts and Membership before the Rush!


Home Brew Forums > Home Brewing Beer > Fermentation & Yeast > Trypan blue?
Reply
 
LinkBack Thread Tools
Old 10-18-2012, 02:56 PM   #1
gandelf
Member
HBT_SUPPORTER.png
Feedback Score: 5 reviews
Recipes 
 
Join Date: Dec 2009
Location: North East, Wisconsin
Posts: 179
Liked 7 Times on 6 Posts
Likes Given: 6

Default Trypan blue?

I have been staining with methylene blue and would like to give trypan blue a
go. Do I use a 0.4% solution (source?) and is the procedure the same as with
methylene? Any other advice would be appreciated.

__________________
gandelf is offline
 
Reply With Quote Quick reply to this message
Old 10-18-2012, 03:09 PM   #2
Johnnyhitch1
HBT_SUPPORTER.png
Feedback Score: 32 reviews
 
Johnnyhitch1's Avatar
Recipes 
 
Join Date: Dec 2011
Location: SiX-ThReE-OnE, NY
Posts: 2,074
Liked 232 Times on 194 Posts
Likes Given: 3419

Default

make sure you add enough aluminum and vent to de-gas

__________________
^~~ "Like" it, Ill Give you beer ~~^
(({Brewing for the Movement Within}))

Primary: Kicking Cans DIPA, ECY20 Golden sour.
Kegged: Conan the Brown, OBS (Oatmeal brett stout)
Aging: Flanders Red w/ ECY02, All out Brett.
BOTTLES:

Cider: Grapfelwine, Apfelwine, Cranfelwine, Applejack
Beer: NZ Brett (BD:9/16/12)
Mead: Blueberry-lemon, Raspberry-Lime, Habenero, POM, Traditional.
Cellar: Maple Whiskey Barrel Stout, ST Pumking Clone
Johnnyhitch1 is offline
 
Reply With Quote Quick reply to this message
Old 10-18-2012, 06:05 PM   #3
gandelf
Member
HBT_SUPPORTER.png
Feedback Score: 5 reviews
Recipes 
 
Join Date: Dec 2009
Location: North East, Wisconsin
Posts: 179
Liked 7 Times on 6 Posts
Likes Given: 6

Default

Could you elaborate on that; or outline the procedure? I'm not a lab tech.

__________________
gandelf is offline
 
Reply With Quote Quick reply to this message
Old 10-18-2012, 06:20 PM   #4
Johnnyhitch1
HBT_SUPPORTER.png
Feedback Score: 32 reviews
 
Johnnyhitch1's Avatar
Recipes 
 
Join Date: Dec 2011
Location: SiX-ThReE-OnE, NY
Posts: 2,074
Liked 232 Times on 194 Posts
Likes Given: 3419

Default

Quote:
Originally Posted by gandelf View Post
Could you elaborate on that; or outline the procedure? I'm not a lab tech.
Nowhere near a lab tech
just a play off breaking bads "blue"
sry i figured someone would be able to answer your question...
__________________
^~~ "Like" it, Ill Give you beer ~~^
(({Brewing for the Movement Within}))

Primary: Kicking Cans DIPA, ECY20 Golden sour.
Kegged: Conan the Brown, OBS (Oatmeal brett stout)
Aging: Flanders Red w/ ECY02, All out Brett.
BOTTLES:

Cider: Grapfelwine, Apfelwine, Cranfelwine, Applejack
Beer: NZ Brett (BD:9/16/12)
Mead: Blueberry-lemon, Raspberry-Lime, Habenero, POM, Traditional.
Cellar: Maple Whiskey Barrel Stout, ST Pumking Clone
Johnnyhitch1 is offline
 
Reply With Quote Quick reply to this message
Old 11-19-2012, 12:24 PM   #5
WoodlandBrew
HBT_SUPPORTER.png
Feedback Score: 0 reviews
 
WoodlandBrew's Avatar
Recipes 
 
Join Date: Oct 2012
Location: Malden, MA
Posts: 1,859
Liked 140 Times on 136 Posts
Likes Given: 59

Default

Bring a dead thread back to life here to answer the question.

Yes, 0.4% Trypan blue works great. Use equal parts of trypan blue and the sample.

__________________

The 2nd edition is now available: Brewing Engineering
Woodland Brewing Research Blog Applied Science for Better Beer.

WoodlandBrew is offline
 
Reply With Quote Quick reply to this message
Old 11-19-2012, 12:50 PM   #6
gandelf
Member
HBT_SUPPORTER.png
Feedback Score: 5 reviews
Recipes 
 
Join Date: Dec 2009
Location: North East, Wisconsin
Posts: 179
Liked 7 Times on 6 Posts
Likes Given: 6

Default

Thanks woodlandbrew, better late than never. I have been working on getting more accurate cell counts. I did a count on a fresh unsmacked pack of wyeast 1056 and got 172,000,000. So, I obviously have some work to do yet.

__________________
gandelf is offline
 
Reply With Quote Quick reply to this message
Old 11-19-2012, 02:11 PM   #7
WoodlandBrew
HBT_SUPPORTER.png
Feedback Score: 0 reviews
 
WoodlandBrew's Avatar
Recipes 
 
Join Date: Oct 2012
Location: Malden, MA
Posts: 1,859
Liked 140 Times on 136 Posts
Likes Given: 59

Default

My brother is a microbiologist, so that's where I learned about trypan blue, but I'm pretty green when it comes to cell counts. 0.4% is the "gold standard" for viability counts of mammalian cells, but it also correlates very well to methylene blue and violet for yeast viability counts.

http://www.coulterflow.com/bciflow/d...20(ta-204).doc

I've never taken apart an un-smacked smack pack, but 176 million does seem low. What are you using for making measurements? Baking measuring cups? Pipette and graduated cylinder?

Have you been counting slurries or starters? I'm curious as to how they correlate to the calculators for different peoples processes.

Have you found a good dilution for methylene blue? White Labs suggests 0.01% which seem much lighter than 0.4% trypan blue. I'm using the 2.303% from a pet store because it is cheap and readily available. 1% seems to be what is sold as a ready to use dilution by chemical companies, but it would be nice to hear from someone doing similar work as to what works for them.

__________________

The 2nd edition is now available: Brewing Engineering
Woodland Brewing Research Blog Applied Science for Better Beer.

WoodlandBrew is offline
 
Reply With Quote Quick reply to this message
Old 11-19-2012, 06:10 PM   #8
gandelf
Member
HBT_SUPPORTER.png
Feedback Score: 5 reviews
Recipes 
 
Join Date: Dec 2009
Location: North East, Wisconsin
Posts: 179
Liked 7 Times on 6 Posts
Likes Given: 6

Default

It should be 172 billion, not million; I was multitasking. At 55 years old, memory is not as reliable has it has been, but I'm not willing to take that lying down.

I have been fiddling around with cell counting and viability for a couple of months now. Two weeks ago I took a weekend course at UWSP; microbiology for brewers. Their was only two homebrewers and about 12 local craftbrewers. It was a great weekend. The professor has been homebrewing for almost 20 years. We did some cell counting and viability. But, the professor's hobby is collecting bacteria and identification, so 95 percent of class time was spent on brewery bugs and there eradication.

I would be interested in exchanging ideas and procedures to improve the reliability and accuracy. I do need to state that I have only been doing this for the past few months.

My brewing process is tight, my fermentation temp control is +/- 1 degree ( liquid, not air contact), so accurate pitch rates are what I'm chasing now.

__________________
gandelf is offline
 
Reply With Quote Quick reply to this message
Reply



Quick Reply
Message:
Options
Thread Tools


Similar Threads
Thread Thread Starter Forum Replies Last Post
Multiple - Blue Balls Belgian Wit (Blue Moon Clone) BierMuncher Belgian and French Ale 565 Yesterday 12:10 AM
All-Grain - Blue ESB 400d English Pale Ale 1 08-20-2012 04:25 PM
Blue, Blue Christmas paint_it_black General Beer Discussion 16 10-04-2010 07:19 PM
For Sale - Tap-a-Draft Blue Ice Tote for blue bottles - MN wookiemofo For Sale 0 09-17-2010 03:36 PM
BM's Blue Balls - Got the Blue, but where's the Balls? jchadscud Recipes/Ingredients 14 04-29-2009 10:20 PM



Newest Threads

LATEST SPONSOR DEALS