I assume you are either making a really big batch or have some old yeast.
Yes you can step up multiple times, or you could just get a couple 1/2 gallon growlers and after the first starter split the next one into multiple vessels for the next step up. This way you can cut out a couple of steps depending on how many alternate vessels you have.
If you think this is a need you will have often ( 8 liters) you might want to pick up a couple 5 liter flasks or 1 gallon growlers.
Originally Posted by Bru
After chilling the starter and pouring off the wort do I need to chill the new wort to the same temp as the original before pouring it in ?
After chilling the starter and pouring off the wort you want to bring the starter to about room temperature. (65-70 deg F) You then want to cool the new wort to within a couple degrees of this same temperature before you pour the yeast in.
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